LOCUS       sequence-60142-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Bedell VM, Wang Y, Campbell JM, Poshusta TL, Starker CG, Krug RG 
            2nd, Tan W, Penheiter SG, Ma AC, Leung AY, Fahrenkrug SC, Carlson 
            DF, Voytas DF, Clark KJ, Essner JJ, Ekker SC
  TITLE     In vivo genome editing using a high-efficiency TALEN system.
  JOURNAL   Nature. 2012 Nov 1;491(7422):114-8. doi: 10.1038/nature11537. Epub 
            2012 Sep 23.
  PUBMED    23000899
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature11537"; journalName: "Nature"; date: "2012-11-1- 1"; 
            volume: "491"; issue: "7422"; pages: "114-8"
COMMENT     Sequence Label: RCIscript-GoldyTALEN-ponzr1L
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             85..105
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             106..132
                     /codon_start=1
                     /product="epitope tag from the baculovirus Autographa 
                     californica GP64 envelope fusion protein"
                     /label=AcV5 tag
                     /translation="SWKDASGWS"
ORIGIN
        1 aacgctcaac tttggcagat ctaactcgag aaagatattg tatatatcgt aacaatagga
       61 ggttcaacaa tggcttcctc ccctccaaag aaaaagagaa aggttagttg gaaggacgca
      121 agtggttggt ctagagtgga tctacgcacg ctcggctaca gtcagcagca gcaagagaag
      181 atcaaaccga aggtgcgttc gacagtggcg cagcaccacg aggcactggt gggccatggg
      241 tttacacacg cgcacatcgt tgcgctcagc caacacccgg cagcgttagg gaccgtcgct
      301 gtcacgtatc agcacataat cacggcgttg ccagaggcga cacacgaaga catcgttggc
      361 gtcggcaaac agtggtccgg cgcacgcgcc ctggaggcct tgctcacgga tgcgggggag
      421 ttgagaggtc cgccgttaca gttggacaca ggccaacttg tgaagattgc aaaacgtggc
      481 ggcgtgaccg caatggaggc agtgcatgca tcgcgcaatg cactgacggg tgcccccctg
      541 aacctgaccc cggaccaagt ggtggctatc gccagcaaca atggcggcaa gcaagcgctc
      601 gaaacggtgc agcggctgtt gccggtgctg tgccaggacc atggcctgac cccggaccaa
      661 gtggtggcta tcgccagcaa cggtggcggc aagcaagcgc tcgaaacggt gcagcggctg
      721 ttgccggtgc tgtgccagga ccatggcctg accccggacc aagtggtggc tatcgccagc
      781 aacaatggcg gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt gctgtgcc
//
