LOCUS       sequence-60143-e         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DHX9A
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..42
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             53..70
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             95..115
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             901..918
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata tgcaccatca
       61 tcatcatcat tcttctggtg tagatctggg taccgagaac ctgtacttcc aatccatgaa
      121 ccaagtgggt gtggttcctt ggtcacctcc acaatccaac tggaatcctt ggactagtag
      181 caacattgat gaggggcctc tggcttttgc tactccagag caaataagca tggacctcaa
      241 gaatgaattg atgtaccagt tggaacagga tcatgatttg caagcaatct tgcaggagag
      301 agagttactg cctgtgaaga aatttgaaag tgagattctg gaagcaatca gccaaaattc
      361 agttgtcatt attagagggg ctactggatg tgggaaaacc acacaggttc cccagttcat
      421 tctagatgac tttatccaga atgaccgagc agcagagtgt aacatcgtag taactcagcc
      481 cagaagaatc agtgcggttt ctgtggcaga gcgagttgca tttgaaagag gagaagagcc
      541 tggaaaaagc tgtggctaca gcgttcgatt tgagtctata cttcctcgtc ctcatgccag
      601 tataatgttt tgtactgtag gtgtgctcct gagaaaatta gaagcaggca ttcgaggaat
      661 cagtcatgta attgtagatg aaatacatga aagagatatt aatactgact tccttttggt
      721 agtactgcgt gatgttgttc aggcttatcc tgaagttcgc attgttctta tgtctgctac
      781 tattgatacc agcatgtttt gtgaatattt cttcaattgc cccatcattg aagtttgaca
      841 gtaaaggtgg atacggatcc gaattcgagc tccgtcgaca agcttgcggc cgcactcgag
      901 caccaccacc accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg
      961 gct
//
