LOCUS       sequence-60165-e         956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 956)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PIK3C2GA
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..42
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             53..70
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             95..115
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             496..513
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(566..584)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      580..627
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(751..770)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata tgcaccatca
       61 tcatcatcat tcttctggtg tagatctggg taccgagaac ctgtacttcc aatccatgtc
      121 gattgaaaga gcaacaattt tagggttcag caagaaatcc agtaatctgt atctgatcca
      181 ggtgacacac agcaacaacg aaacaagcct gacagaaaaa tcatttgagc agttttcaaa
      241 acttcacagc caacttcaga agcagtttgc atcactgact ctcccagagt ttcctcattg
      301 gtggcaccta ccttttacaa attcagatca cagaagattc agagatctaa atcattacat
      361 ggaacagata ttaaatgtat cacatgaagt tacaaacagt gattgtgtac ttagcttttt
      421 cctctctgag tgacagtaaa ggtggatacg gatccgaatt cgagctccgt cgacaagctt
      481 gcggccgcac tcgagcacca ccaccaccac cactgagatc cggctgctaa caaagcccga
      541 aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc
      601 tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg attggcgaat
      661 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
      721 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      781 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      841 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      901 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttcttt
//
