LOCUS       sequence-60166-e         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PARP2A
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..42
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             53..70
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             95..115
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(759..770)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata tgcaccatca
       61 tcatcatcat tcttctggtg tagatctggg taccgagaac ctgtacttcc aatccatgga
      121 tcttcgggta caggagttaa taaagttgat ctgtaatgtt caggccatgg aagaaatgat
      181 gatggaaatg aagtataata ccaagaaagc cccacttggg aagctgacag tggcacaaat
      241 caaggcaggt taccagtctc ttaagaagat tgaggattgt attcgggctg gccagcatgg
      301 acgagctctc atggaagcat gcaatgaatt ctacaccagg attccgcatg actttggact
      361 ccgtactcct ccactaatcc ggacacagaa ggaactgtca gaaaaaatac aattactaga
      421 ggctttggga gacattgaaa ttgctattaa gctggtgaaa acagagctac aaagcccaga
      481 acacccattg gaccaacact atagaaacct acattgtgcc ttgcgccccc ttgaccatga
      541 aagttatgag ttcaaagtga tttcccagta cctacaatct acccatgctc ccacacacag
      601 cgactatacc atgaccttgc tggatttgtt tgaagtggag aaggatggtg agaaagaagc
      661 cttcagagag gaccttcata acaggatgct tctatggcat ggttccagga tgagtaactg
      721 ggtgggaatc ttgagccatg ggcttcgaat tgcccaccct gaagctccca tcacaggtta
      781 catgtttggg aaaggaatct actttgctga catgtcttcc aagagtgcca attactgctt
      841 tgcctctcgc ctaaagaata caggactgct gctcttatca gaggtagctc taggtcagtg
      901 taatgaacta ctagaggcca atcctaaggc cgaaggattg cttcaaggta aacatagcac
      961 caaggggctg gg
//
