LOCUS       sequence-60167-e         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ACOT4A
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..42
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             53..70
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             95..115
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata tgcaccatca
       61 tcatcatcat tcttctggtg tagatctggg taccgagaac ctgtacttcc aatccatgtc
      121 agcaacgctg atcctggagc ccccaggccg ctgctgctgg aacgagccgg tgcgcattgc
      181 cgtgcgcggc ctggccccgg agcagcgggt tacgctgcgc gcgtccctgc gcgacgagaa
      241 gggcgcgctc ttccgggccc acgcgcgcta ctgcgccgac gcctgcggcg agctggacct
      301 ggagcgcgca cccgcgctgg gcggcagctt cgcgggactc gagcccatgg ggctgctctg
      361 ggccctggaa cccgagaagc ctttttggcg cttcctgaag cgggacgtac agattccttt
      421 tgtcgtggag ttggaggtgc tggacggcca cgaccccgag cctggacggc tgctgtgcca
      481 ggcgcagcac gagcgccact tcctcccgcc aggggtgtgg cgccagtcgg tgcgagcggg
      541 ccgggtgcgc gccacgctct tcctgccgcc aggacctgga cccttcccag ggatcattga
      601 catctttggt attggagggg gcctcttgga atatcgagcc agcctccttg ctggccatgg
      661 ctttgccacg ttggctctag cttattataa ctttgaagat ctccccaata acatggacaa
      721 cntatccctg gagtacttcg aagaagccgt atgctacatg cttcaacatc cccaggtaaa
      781 aggcccaggc attgggcttt tgggcatttc tctaggagct gatatttgtc tctcaatggc
      841 ctcattcttg aagaatgtct cagccacagt ttccatcaat ggatctggga tcagtgggaa
      901 cacagccatc aactataagc acagtagcat t
//
