LOCUS       sequence-60168-e         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: HSPA5A
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..42
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             53..70
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             95..115
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata tgcaccatca
       61 tcatcatcat tcttctggtg tagatctggg taccgagaac ctgtacttcc aatccatgga
      121 cgtgggcacg gtggtcggca tcgacctggg gaccacctac tcctgcgtcg gcgtgttcaa
      181 gaacggccgc gtggagatca tcgccaacga tcagggcaac cgcatcacgc cgtcctatgt
      241 cgccttcact cctgaagggg aacgtctgat tggcgatgcc gccaagaacc agctcacctc
      301 caaccccgag aacacggtct ttgacgccaa gcggctcatc ggccgcacgt ggaatgaccc
      361 gtctgtgcag caggacatca agttcttgcc gttcaaggtg gttgaaaaga aaactaaacc
      421 atacattcaa gttgatattg gaggtgggca aacaaagaca tttgctcctg aagaaatttc
      481 tgccatggtt ctcactaaaa tgaaagaaac cgctgaggct tatttgggaa agaaggttac
      541 ccatgcagtt gttactgtac cagcctattt taatgatgcc caacgccaag caaccaaaga
      601 cgctggaact attgctggcc taaatgttat gaggatcatc aacgagccta cggcagctgc
      661 tattgcttat ggcctggata agagggaggg ggagaagaac atcctggtgt ttgacctggg
      721 tggcggaacc ttcgatgtgt ctcttctcac cattgacaat ggtgtcttcg aagttgtggc
      781 cactaatgga gatactcatc tgggtggaga agactttgac cagcgtgtca tggaacactt
      841 catcaaactg tacaaaaaga agacgggcaa agatgtcagg aaagacaata gagctgtgca
      901 gaaactccgg cgcgaggtag aaaaggccaa acgggccctg tcttctcagc atca
//
