LOCUS       sequence-60170-e         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DDX53A
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..42
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             53..70
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             95..115
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata tgcaccatca
       61 tcatcatcat tcttctggtg tagatctggg taccgagaac ctgtacttcc aatccatgac
      121 gtgtgatgac ttgaaaagtg gtgaaaagcg tctcattcca aaaccaactt gcaggtttaa
      181 agacgctttt cagcaatacc ctgatcttct gaaaagcata ataagggtag ggattttaaa
      241 gccaacgcca attcagtcac aggcatggcc aattattcta caaggaatag atcttatagt
      301 agttgcacaa accggaacag ggaaaacatt gtcctatcta atgcctgggt ttattcatct
      361 tgattctcaa ccaatatcta gagagcaaag gaatgggcct gggatgctag tccttacacc
      421 cactagagag ttggctcttc acgtggaagc tgaatgttca aagtattcat ataaaggtct
      481 caaaagcatt tgcatatatg gtggtagaaa cagaaatgga caaatagaag acattagcaa
      541 aggtgtagat atcattattg caactcctgg gaggctgaat gacctacaaa tgaataactc
      601 tgtcaaccta agaagcataa cctacttggt tatagatgag gcagataaaa tgctggatat
      661 ggaatttgaa ccccagataa ggaagatttt attagatgtg cgcccagacc gacagactgt
      721 tatgacaagt gcaacttggc cagatactgt acgtcaacta gcactttctt atttgaaaga
      781 tcctatgatt gtttatgttt gacagtaaa
//
