LOCUS       sequence-60171-e         979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 979)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SNX7BA
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(39..56)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(106..123)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             559..581
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    596..620
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(620..639)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(621..639)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(723..742)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tcagcttcct ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg
       61 agtgcggccg caagcttgtc gacggagctc gaattcggat cctcaatggt ggtgatgatg
      121 gtgcgcgtga gaagagagtt cccaagcttg tgcagtgaga aaaattttga agtcttcatt
      181 aaatgttaaa gttggatgat cagcaattcg gttcaaaaat ttatgtaaag ccttcctgcg
      241 tgtctcaatg aagtcatcgt taaagcgttc caccattcct tttactataa acttttctgg
      301 caatggtgga ataatcagag tggggtgtgc ttcttccagt tttcccttca accaaaggaa
      361 atcttgatat cgtctcctaa cttcaaattc actggagtca aattccccac gagatgtctt
      421 agtaataatc ctatacgtaa tgaaagtttc tattgtagta acatgacttt caggttcatc
      481 aactgtgatg aagagatcct ttaaatctgg ttcatccatt aattatctcc tctttgtgat
      541 tcatgattca tatgtatatc tccttcttaa agttaaacaa aattatttct agaggggaat
      601 tgttatccgc tcacaattcc cctatagtga gtcgtattaa tttcgcggga tcgagatctc
      661 gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg
      721 cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag
      781 cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat
      841 ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg
      901 ctgcttccta atgcaggagt cgcataaggg agagcgtcga gatcccggac accatcgaat
      961 ggcgcaaaac ctttcgcgg
//
