LOCUS       sequence-60175-e         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: UMPSA
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(39..56)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(106..123)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             760..782
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    797..821
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(821..840)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(822..840)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(924..943)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tcagcttcct ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg
       61 agtgcggccg caagcttgtc gacggagctc gaattcggat cctcaatggt ggtgatgatg
      121 gtgcgcctcc tgaataaacc tcttcactct cccaactgtc tcagcatcaa cttttttctg
      181 ctgctcgaga atctccagca ttttggacaa tgtacacact gagtggaggc ggatcccgtg
      241 cgcctgcaac ttgtccttgc ctccctgctc tctgtccaac agcactatgg catcagtgac
      301 cttcaagccc tccttctgaa gaacctcaac agtttccaaa acactagatc cactggtgac
      361 aacatcttca atgattaaac aggtttctcc tggattaata gttccttcta caagacgctt
      421 agttccataa tcctttgttt ctttccttct aataagcatt ggaatttgat tggttgaaca
      481 gataactgta gccaatggca aagctgtata aggcactcca cacacggtgt caaaactgat
      541 gcctgcattt tgggcagttt ggaataaaat atctgcaacc tgactcagaa gacgcggtcg
      601 agacacgatg ccccgcagat cgatgtagat gggggaggaa agcccgctct tcagcacgaa
      661 gtccccaaac ttgaaagcct gcacgtcgta cagacccgtc accaatggcc ccaaagccat
      721 taattatctc ctctttgtga ttcatgattc atatgtatat ctccttctta aagttaaaca
      781 aaattatttc tagaggggaa ttgttatccg ctcacaattc ccctatagtg agtcgtatta
      841 atttcgcggg atcgagatct cgatcctcta cgccggacgc atcgtggccg gcatcaccgg
      901 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc
//
