LOCUS       sequence-60176-e         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: LASP1A
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..42
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             53..70
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             95..115
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             373..390
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(443..461)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      457..504
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      541..921
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(628..647)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     838..859
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata tgcaccatca
       61 tcatcatcat tcttctggtg tagatctggg taccgagaac ctgtacttcc aatccatggc
      121 cccaggtggt ggcgggaagc ggtaccgcgc ggtgtatgac tacagcgccg ccgacgagga
      181 cgaggtctcc ttccaggacg gggacaccat cgtcaacgtg cagcagatcg acgacggctg
      241 gatgtacggg acggtggagc gcaccggcga cacggggatg ctgccggcca actacgtgga
      301 ggccatttga cagtaaaggt ggatacggat ccgaattcga gctccgtcga caagcttgcg
      361 gccgcactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag
      421 gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct
      481 aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatt ggcgaatggg
      541 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
      601 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      661 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      721 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      781 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      841 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      901 aaggaatttt gccgatttcg gcctattggt tga
//
