LOCUS       sequence-60180-e         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: TBSAHA
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..42
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             53..70
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             95..115
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata tgcaccatca
       61 tcatcatcat tcttctggtg tagatctggg taccgagaac ctgtacttcc aatccatgta
      121 caaggtacgt gatatcagcc tcgctgaatg gggccgcagg gagcttgagc tcgcagagaa
      181 cgaaatgccg ggtctcatgg aacttcgccg tgagtacggc ccttcgaagc ctctgaaggg
      241 tgccaagatc gctggctgcc tgcacatgac tatgcagact gctgtgctta tcgagacgct
      301 tgtggagctc ggtgctgaag tgcggtgggc ctcgtgcaac atcttctcca cacaggacca
      361 tgcagccgcc gcgattgcga agcggggtat ccctgttttt gcgtggaagg gcgaaactga
      421 agaggagtac atgtggtgta tgaagcagac actgaagggc ttcagcggtg atggttaccc
      481 caacatgttg ctcgacgacg gtggcgacct gacaaattac gtcctggatg agtgcaagga
      541 gctggacgga aaaatatacg gtgtctccga ggagacaacg acaggtgtaa aaaacctgta
      601 caagcggctg cagcggggta agctgaccat ccccgccatg aatgtgaacg acagtgtgac
      661 gaagagcaag ttcgacaatc tgtacggttg ccgtgagtct ctcgtggacg gcatcaagcg
      721 tgctaccgat gtaatgattg ctggcaagac cgcatgtgtc tgtggatatg gtgacgtggg
      781 caaaggttgc gcggctgcgc tgcgtggctt tggagcacgt gtcgttgtga cggaggttga
      841 tccaattaat gcgctgca
//
