LOCUS       sequence-60181-e         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: NUDT6A
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             53..70
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             95..115
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             712..729
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(782..800)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 attcccctct agaaataatt ttgtttaact ttaagatgga gatatacata tgcaccatca
       61 tcatcatcat tcttctggtg tagatctggg taccgagaac ctgtacttcc aatccatgtc
      121 acatcaagta ggagttgcag gagctgtatt tgatgaaagt actagaaaaa tactggttgt
      181 acaagatcga aataaattga aaaatatgtg gaagtttcca ggaggcctgt cagagcctga
      241 agaagatatt ggagacacag cggttcgaga agtttttgaa gagactggta taaaatcaga
      301 attcaggtcc gtcctgagta ttcggcaaca gcacacaaat cctggagctt ttgggaagtc
      361 agatatgtat atcatctgcc gcctaaagcc atattcattc accataaatt tttgccagga
      421 agaatgctta agatgtgagt ggatggatct caatgacctg gcgaagactg aaaatacaac
      481 tcccatcacc agcagagttg ctaggctgct gctgtatggg tacagagaag ggtttgacaa
      541 aattgacctg actgtggaag aacttccagc agtttacaca ggactgtttt ataaactcta
      601 tcataaggaa ctgccagaga attataaaac tatgaaagga attgattaac agtaaaggtg
      661 gatacggatc cgaattcgag ctccgtcgac aagcttgcgg ccgcactcga gcaccaccac
      721 caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc
      781 accgctgagc aataactagc ataa
//
