LOCUS       sequence-60198-e        8746 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8746)
  AUTHORS   Dolt KS, Lawrence ML, Miller-Hodges E, Slight J, Thornburn A, 
            Devenney PS, Hohenstein P
  TITLE     A universal vector for high-efficiency multi-fragment recombineering
            of BACs and knock-in constructs.
  JOURNAL   PLoS One. 2013 Apr 29;8(4):e62054. doi: 
            10.1371/journal.pone.0062054. Print 2013.
  PUBMED    23637962
REFERENCE   2  (bases 1 to 8746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-04-29"; volume: "8(4):e62054. doi"; pages: " 
            10.1371/journal.pone.0062054. Print 2013"
COMMENT     Sequence Label: pDEST-IRES-puro-polyA
FEATURES             Location/Qualifiers
     source          1..8746
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(33..621)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(113..132)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(792..1583,1584..1652))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1415..1434
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1653..1757)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1783..2238)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1920..1941)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2132..2151
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2365..2387
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2380..2396
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2403..2422
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2403..2421
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2483..2499
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2484..2500
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2517..2641
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(3037..3342)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(3139..3158)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(3662..4345)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     primer_bind     4260..4279
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(4346..4448)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    4593..4688
                     /gene="mutant version of attL"
                     /label=attL5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    4727..5188
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4783..4800)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5010..5029
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     protein_bind    complement(5195..5215)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             5221..5820
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5221..5240)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5717..5737
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    5822..5842
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     polyA_signal    5845..6052
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(6069..6093)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     6108..6124
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6126..6159
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    6164..6197
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     promoter        6205..6704
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6240..6263)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6637..6656
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        6716..6763
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6782..7585
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6845..6864)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7455..7474
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7623..7847
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    7925..8149
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8240..8256)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8257..8273)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8257..8273)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(8338..8356)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8377..8393)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8390..8412)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8401..8417
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8425..8431,8432..8449,8450..8455))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8470..8491
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8608..8625)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
       61 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
      121 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
      181 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
      241 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
      301 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
      361 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
      421 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
      481 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
      541 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
      601 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
      661 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
      721 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
      781 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
      841 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
      901 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
      961 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     1021 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     1081 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     1141 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     1201 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     1261 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     1321 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     1381 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     1441 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     1501 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     1561 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     1621 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     1681 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     1741 caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata
     1801 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     1861 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     1921 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     1981 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     2041 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     2101 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     2161 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     2221 cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     2281 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     2341 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat
     2401 tgtaatacga ctcactatag ggcgaattgg gtaccgggcc ccccctcgaa gatctcatat
     2461 ggtttaaaca cgcgtgctag catcgaggtc gacggtatcg ataagcttga tatcaaacaa
     2521 gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt
     2581 agattttgca taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat
     2641 ggtcgaccga cagccttcca aatgttcttc gggtgatgct gccaacttag tcgaccgaca
     2701 gccttccaaa tgttcttctc aaacggaatc gtcgtatcca gcctactcgc tattgtcctc
     2761 aatgccgtat taaatcataa aaagaaataa gaaaaagagg tgcgagcctc ttttttgtgt
     2821 gacaaaataa aaacatctac ctattcatat acgctagtgt catagtcctg aaaatcatct
     2881 gcatcaagaa caatttcaca actcttatac ttttctctta caagtcgttc ggcttcatct
     2941 ggattttcag cctctatact tactaaacgt gataaagttt ctgtaatttc tactgtatcg
     3001 acctgcagac tggctgtgta taagggagcc tgacatttat attccccaga acatcaggtt
     3061 aatggcgttt ttgatgtcat tttcgcggtg gctgagatca gccacttctt ccccgataac
     3121 ggagaccggc acactggcca tatcggtggt catcatgcgc cagctttcat ccccgatatg
     3181 caccaccggg taaagttcac gggagacttt atctgacagc agacgtgcac tggccagggg
     3241 gatcaccatc cgtcgcccgg gcgtgtcaat aatatcactc tgtacatcca caaacagacg
     3301 ataacggctc tctcttttat aggtgtaaac cttaaactgc atttcaccag cccctgttct
     3361 cgtcagcaaa agagccgttc atttcaataa accgggcgac ctcagccatc ccttcctgat
     3421 tttccgcttt ccagcgttcg gcacgcagac gacgggcttc attctgcatg gttgtgctta
     3481 ccagaccgga gatattgaca tcatatatgc cttgagcaac tgatagctgt cgctgtcaac
     3541 tgtcactgta atacgctgct tcatagcata cctctttttg acatacttcg ggtatacata
     3601 tcagtatata ttcttatacc gcaaaaatca gcgcgcaaat acgcatactg ttatctggct
     3661 tttagtaagc cggatccacg cggcgtttac gccccccctg ccactcatcg cagtactgtt
     3721 gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa
     3781 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg
     3841 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
     3901 gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
     3961 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
     4021 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
     4081 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacggaat tccggatgag
     4141 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct
     4201 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag
     4261 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg
     4321 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact
     4381 caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt
     4441 gccgatcaac gtctcatttt cgccaaaagt tggcccaggg cttcccggta tcaacaggga
     4501 caccaggatt tatttattct gcgaagtgat cttccgtcac aggtatttat tcggcgcaaa
     4561 gtgcgtcggg tgatgctgcc aacttagtcg acaaataatg attttatttt gactgatagt
     4621 gacctgttcg ttgcaacaaa ttgatgagca atgctttttt ataatgccaa ctttgtatac
     4681 aaaagttgtg gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     4741 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
     4801 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     4861 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     4921 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     4981 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     5041 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
     5101 gctttacatg tgtttagtcg aggttaaaaa acgtctaggc cccccgaacc acggggacgt
     5161 ggttttcctt tgaaaaacac gatgataata cacccaactt ttctatacaa agttgctacc
     5221 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta
     5281 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     5341 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     5401 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     5461 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     5521 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     5581 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     5641 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     5701 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     5761 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     5821 acaactttgt ataataaagt tgctctgtgc cttctagttg ccagccatct gttgtttgcc
     5881 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     5941 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     6001 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     6061 gctctattac ccagctttct tgtacaaagt ggttgatatc gaattctgta aaacgacggc
     6121 cagtgataac ttcgtataat gtatgctata cgaagttatt caggaagttc ctattcttca
     6181 aatagtatag gaacttcatc taccgggtag gggaggcgct tttcccaagg cagtctggag
     6241 catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca
     6301 cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc
     6361 caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca
     6421 ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg
     6481 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt
     6541 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg
     6601 gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca
     6661 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agcctgttga
     6721 caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac
     6781 catgggatcg gccattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     6841 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     6901 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     6961 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     7021 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     7081 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     7141 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     7201 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     7261 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     7321 catgcccgac ggcgatgatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     7381 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     7441 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     7501 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     7561 tcgccttctt gacgagttct tctgagggga tcaattctct agagctcgct gatcagcctc
     7621 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     7681 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     7741 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     7801 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     7861 aagaaccagc tggggctcga ctagagcttg cggaaccctt ctagagctcg ctgatcagcc
     7921 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     7981 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     8041 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     8101 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     8161 gaaagaacca gctggggctc gactagagct tgcggaaccc ttcgaagttc ctattcttca
     8221 aatagtatag gaactcatgg tcatagctgt ttcctggatc cactagttct agagcggcct
     8281 taattaaatt taaatggcgc gccggccgcc accgcggtgg agctccagct tttgttccct
     8341 ttagtgaggg ttaatttcga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     8401 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     8461 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     8521 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     8581 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     8641 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     8701 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggcc
//
