LOCUS       sequence-60205-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Activation Domain: VP64; RVD of C-terminal 0.5 TALE repeat: NN 
            (binds G) .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Maeder ML, Linder SJ, Reyon D, Angstman JF, Fu Y, Sander JD, Joung 
            JK
  TITLE     Robust, synergistic regulation of human gene expression using TALE 
            activators.
  JOURNAL   Nat Methods. 2013 Feb 10. doi: 10.1038/nmeth.2366.
  PUBMED    23396285
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Feb 10. doi: 10.1038/nmeth.2366."
COMMENT     Sequence Label: MLM3587
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      14..23
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             26..46
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tcgtgaagcg gccgccacca tggcccccaa gaagaagagg aaggtgggca ttcaccgcgg
       61 ggtacctatg gtggacttga ggacactcgg ttattcgcaa cagcaacagg agaaaatcaa
      121 gcctaaggtc aggagcaccg tcgcgcaaca ccacgaggcg cttgtggggc atggcttcac
      181 tcatgcgcat attgtcgcgc tttcacagca ccctgcggcg cttgggacgg tggctgtcaa
      241 ataccaagat atgattgcgg ccctgcccga agccacgcac gaggcaattg taggggtcgg
      301 taaacagtgg tcgggagcgc gagcacttga ggcgctgctg actgtggcgg gtgagcttag
      361 ggggcctccg ctccagctcg acaccgggca gctgctgaag atcgcgaaga gagggggagt
      421 aacagcggta gaggcagtgc acgcctggcg caatgcgctc accggggccc ccttgaacag
      481 agacgatcta gagctagacg tctcgctgac acccgaacag gtggtcgcca ttgctaataa
      541 taacggagga cggccagcct tggagagcat tgtggcccag ctgtccaggc cggaccctgc
      601 cctggccgcg ttaaccaacg accacctcgt cgccttggcc tgcctcggcg gacgtcctgc
      661 gctggatgca gtgaaaaagg gattgccgca cgcgccggcc ttgatcaaaa gaaccaatcg
      721 ccgtattccc gaacgcacat cccatcgcgt tgccgaccac gcgcaagtgg ttcgcgtgct
      781 gggttttttc cagtgccact cccacccagc gcaagcattt gatgacgcca tgacgcagtt
      841 cgggatgagc ggag
//
