LOCUS       sequence-60206-e         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Activation Domain: VP64; RVD of C-terminal 0.5 TALE repeat: NG 
            (binds T) .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Maeder ML, Linder SJ, Reyon D, Angstman JF, Fu Y, Sander JD, Joung 
            JK
  TITLE     Robust, synergistic regulation of human gene expression using TALE 
            activators.
  JOURNAL   Nat Methods. 2013 Feb 10. doi: 10.1038/nmeth.2366.
  PUBMED    23396285
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Feb 10. doi: 10.1038/nmeth.2366."
COMMENT     Sequence Label: MLM3583
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      15..24
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             27..47
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gtcgtgaagc ggccgccacc atggccccca agaagaagag gaaggtgggc attcaccgcg
       61 gggtacctat ggtggacttg aggacactcg gttattcgca acagcaacag gagaaaatca
      121 agcctaaggt caggagcacc gtcgcgcaac accacgaggc gcttgtgggg catggcttca
      181 ctcatgcgca tattgtcgcg ctttcacagc accctgcggc gcttgggacg gtggctgtca
      241 aataccaaga tatgattgcg gccctgcccg aagccacgca cgaggcaatt gtaggggtcg
      301 gtaaacagtg gtcgggagcg cgagcacttg aggcgctgct gactgtggcg ggtgagctta
      361 gggggcctcc gctccagctc gacaccgggc agctgctgaa gatcgcgaag agagggggag
      421 taacagcggt agaggcagtg cacgcctggc gcaatgcgct caccggggcc cccttgaaca
      481 gagacgatct agagctagac gtctcgctga cacccgaaca ggtggtcgcc attgctagca
      541 acgggggagg acggccagcc ttggagagca ttgtggccca gctgtccagg ccggaccctg
      601 ccctggccgc gttaaccaac gaccacctcg tcgccttggc ctgcctcggc ggacgtcctg
      661 cgctggatgc agtgaaaaag ggattgccgc acgcgccggc cttgatcaaa agaaccaatc
      721 gccgtattcc cgaacgcaca tcccatcgcg ttgccgacca cgcgcaagtg gttcgcgtgc
      781 tgggtttttt ccagtgccac tcc
//
