LOCUS       sequence-60209-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Activation Domain: NF-kB; RVD of C-terminal 0.5 TALE repeat: NN 
            (binds G) .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Maeder ML, Linder SJ, Reyon D, Angstman JF, Fu Y, Sander JD, Joung 
            JK
  TITLE     Robust, synergistic regulation of human gene expression using TALE 
            activators.
  JOURNAL   Nat Methods. 2013 Feb 10. doi: 10.1038/nmeth.2366.
  PUBMED    23396285
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Feb 10. doi: 10.1038/nmeth.2366."
COMMENT     Sequence Label: MLM2585
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             30..50
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggtgtcgtga agcggccgcc accatggccc ccaagaagaa gaggaaggtg ggcattcacc
       61 gcggggtacc tatggtggac ttgaggacac tcggttattc gcaacagcaa caggagaaaa
      121 tcaagcctaa ggtcaggagc accgtcgcgc aacaccacga ggcgcttgtg gggcatggct
      181 tcactcatgc gcatattgtc gcgctttcac agcaccctgc ggcgcttggg acggtggctg
      241 tcaaatacca agatatgatt gcggccctgc ccgaagccac gcacgaggca attgtagggg
      301 tcggtaaaca gtggtcggga gcgcgagcac ttgaggcgct gctgactgtg gcgggtgagc
      361 ttagggggcc tccgctccag ctcgacaccg ggcagctgct gaagatcgcg aagagagggg
      421 gagtaacagc ggtagaggca gtgcacgcct ggcgcaatgc gctcaccggg gcccccttga
      481 acagagacga tctagagcta gacgtctcgc tgacacccga acaggtggtc gccattgcta
      541 ataataacgg aggacggcca gccttggaga gcattgtggc ccagctgtcc aggccggacc
      601 ctgccctggc cgcgttaacc aacgaccacc tcgtcgcctt ggcctgcctc ggcggacgtc
      661 ctgcgctgga tgcagtgaaa aagggattgc cgcacgcgcc ggccttgatc aaaagaacca
      721 atcgccgtat tcccgaacgc acatcccatc gcgttgccga ccacgcgcaa gtggttcgcg
      781 tgctgggttt tttccagtgc cactcccacc cagcgcaagc atttgatgac g
//
