LOCUS       sequence-60221-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, 
            Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O
  TITLE     A Versatile Toolkit to Produce Sensitive FRET Biosensors to 
            Visualize Signaling in Time and Space.
  JOURNAL   Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.
  PUBMED    23882122
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-23"; volume: "6(285):rs12. doi"; pages: " 
            10.1126/scisignal.2004135"
COMMENT     Sequence Label: EKAR2G_design1_mTFP_175_Venus_173
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(40..63)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(join(133..645,646..648,649..651))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     326..345
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     585..606
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(709..730)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccttctgata ggcagcctgc acctgaggtt aatcacttag tgatggtgat ggtgatggtg
       61 gtgctcgagt cattagatat ctcattcgtc aagttcaagt tcttcaagtt ttttttgaag
      121 gatatcggta ccctcgatgt tgtggcggat cttgaagttg gccttgatgc cgttcttctg
      181 cttgtcggcg gtgatataga cgttgtggct gttgtagttg tactccagct tgtgccccag
      241 gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc
      301 gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt
      361 gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg
      421 gtcggggtag cgggcgaagc acatcaggcc gtagcccagg gtggtcacga gggtgggcca
      481 gggcacgggc agcttgccgg tggtgcagat cagcttcagg gtcagcttgc cgtaggtggc
      541 atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc
      601 gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tgccaccgct
      661 gccacccttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag
      721 caggaccatg tgatcgcgct tctcgttggg gtctttgctc agggcggact ggtagctcag
      781 gtagtggttg tcgggcagca gcacggggcc gtcgccgatg gggg
//
