LOCUS       sequence-60226-e         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Activation Domain: VP64; RVD of C-terminal 0.5 TALE repeat: HD 
            (binds C).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Maeder ML, Linder SJ, Reyon D, Angstman JF, Fu Y, Sander JD, Joung 
            JK
  TITLE     Robust, synergistic regulation of human gene expression using TALE 
            activators.
  JOURNAL   Nat Methods. 2013 Feb 10. doi: 10.1038/nmeth.2366.
  PUBMED    23396285
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Feb 10. doi: 10.1038/nmeth.2366."
COMMENT     Sequence Label: MLM3585
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(10..29)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     64..83
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(125..274)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
ORIGIN
        1 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
       61 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaatta
      121 attataacat atcgagatcg aaatcgtcca gagcatcgga gccgagcatg tccagatcaa
      181 agtcatccaa tgcgtcgctt cccaacatat ccaggtcaaa gtcatcgagg gcatcagaac
      241 ccagcatgtc gagatcgaaa tcgtccagcg cgtcggcgcg cccggatcct cctccgctca
      301 tcccgaactg cgtcatggcg tcatcaaatg cttgcgctgg gtgggagtgg cactggaaaa
      361 aacccagcac gcgaaccact tgcgcgtggt cggcaacgcg atgggatgtg cgttcgggaa
      421 tacggcgatt ggttcttttg atcaaggccg gcgcgtgcgg caatcccttt ttcactgcat
      481 ccagcgcagg acgtccgccg aggcaggcca aggcgacgag gtggtcgttg gttaacgcgg
      541 ccagggcagg gtccggcctg gacagctggg ccacaatgct ctccaaggct ggccgtcctc
      601 cgtcgtggga agcaatggcg accacctgtt cgggtgtcag cgagacgtct agctctagat
      661 cgtctctgtt caagggggcc ccggtgagcg cattgcgcca ggcgtgcact gcctctaccg
      721 ctgttactcc ccctctcttc gcgatcttca gcagctgccc ggtgtcgagc tggagcggag
      781 gccccctaag ct
//
