LOCUS       sequence-60227-e         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Activation Domain: VP64; RVD of C-terminal 0.5 TALE repeat: NN 
            (binds G) .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Maeder ML, Linder SJ, Reyon D, Angstman JF, Fu Y, Sander JD, Joung 
            JK
  TITLE     Robust, synergistic regulation of human gene expression using TALE 
            activators.
  JOURNAL   Nat Methods. 2013 Feb 10. doi: 10.1038/nmeth.2366.
  PUBMED    23396285
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Feb 10. doi: 10.1038/nmeth.2366."
COMMENT     Sequence Label: MLM3587
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(5..24)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     59..78
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(120..269)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
ORIGIN
        1 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
       61 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aattaattat
      121 aacatatcga gatcgaaatc gtccagagca tcggagccga gcatgtccag atcaaagtca
      181 tccaatgcgt cgcttcccaa catatccagg tcaaagtcat cgagggcatc agaacccagc
      241 atgtcgagat cgaaatcgtc cagcgcgtcg gcgcgcccgg atcctcctcc gctcatcccg
      301 aactgcgtca tggcgtcatc aaatgcttgc gctgggtggg agtggcactg gaaaaaaccc
      361 agcacgcgaa ccacttgcgc gtggtcggca acgcgatggg atgtgcgttc gggaatacgg
      421 cgattggttc ttttgatcaa ggccggcgcg tgcggcaatc cctttttcac tgcatccagc
      481 gcaggacgtc cgccgaggca ggccaaggcg acgaggtggt cgttggttaa cgcggccagg
      541 gcagggtccg gcctggacag ctgggccaca atgctctcca aggctggccg tcctccgtta
      601 ttattagcaa tggcgaccac ctgttcgggt gtcagcgaga cgtctagctc tagatcgtct
      661 ctgttcaagg gggccccggt gagcgcattg cgccaggcgt gcactgcctc taccgctgtt
      721 actccccctc tcttcgcgat cttcagcagc tgcccggtgt cgagctgga
//
