LOCUS       sequence-60229-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Activation Domain: NF-kB; RVD of C-terminal 0.5 TALE repeat: NI 
            (binds A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Maeder ML, Linder SJ, Reyon D, Angstman JF, Fu Y, Sander JD, Joung 
            JK
  TITLE     Robust, synergistic regulation of human gene expression using TALE 
            activators.
  JOURNAL   Nat Methods. 2013 Feb 10. doi: 10.1038/nmeth.2366.
  PUBMED    23396285
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Feb 10. doi: 10.1038/nmeth.2366."
COMMENT     Sequence Label: MLM2581
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(5..24)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     59..78
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(120..491)
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
ORIGIN
        1 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
       61 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aattaattaa
      121 gagctgatct gactcagcag ggctgagaag tccatgtccg caatggagga gaagtcttca
      181 tctcctgaaa ggaggccatt ggggagcccc ggggccccca gtggagcagg agctgggtcg
      241 gggggcctct gggcccctgt cactaggcga gttatagcct cagggtactc catcagcatg
      301 ggctcagttg tgtggggggc cacaggtatg ccctggttca gcagctgctg aaactcggag
      361 ttatcgacgg atgccaggtc tgtgaacaca gctgggtctg tgctgttgcc aagcaaggcc
      421 cccaggtctt catcatcaaa ctgcagctgc agcagggcct ctgacagcgt tccttcccca
      481 gcctgggtgg gcttgggggc aggtggggcc acagcctgag gagggcctgg ggctaggact
      541 gggacagggg ctggggcctg ggccagagct gataccatgg ctggagcagg ggcaggggct
      601 ggagcctggg gcaggacttg gggaggggcc ggggccaagg ccgaggcctg gctgatctgc
      661 ccagaaggaa acaccatggt gggaaactca tcatagttga tggtgctcag ggatgacgta
      721 aagggatagg gctggggtgc tggcttgggg acagaagctg agctgcggga aggcacagca
      781 atgcgtcgag
//
