LOCUS       sequence-60232-e         572 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Activation Domain: NF-kB; RVD of C-terminal 0.5 TALE repeat: NG 
            (binds T) .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Maeder ML, Linder SJ, Reyon D, Angstman JF, Fu Y, Sander JD, Joung 
            JK
  TITLE     Robust, synergistic regulation of human gene expression using TALE 
            activators.
  JOURNAL   Nat Methods. 2013 Feb 10. doi: 10.1038/nmeth.2366.
  PUBMED    23396285
REFERENCE   2  (bases 1 to 572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Feb 10. doi: 10.1038/nmeth.2366."
COMMENT     Sequence Label: MLM2579
FEATURES             Location/Qualifiers
     source          1..572
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(12..31)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     66..85
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(127..498)
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
ORIGIN
        1 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
       61 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaat
      121 taattaagag ctgatctgac tcagcagggc tgagaagtcc atgtccgcaa tggaggagaa
      181 gtcttcatct cctgaaagga ggccattggg gagccccggg gcccccagtg gagcaggagc
      241 tgggtcgggg ggcctctggg cccctgtcac taggcgagtt atagcctcag ggtactccat
      301 cagcatgggc tcagttgtgt ggggggccac aggtatgccc tggttcagca gctgctgaaa
      361 ctcggagtta tcgacggatg ccaggtctgt gaacacagct gggtctgtgc tgttgccaag
      421 caaggccccc aggtcttcat catcaaactg cagctgcagc agggcctctg acagcgttcc
      481 ttccccagcc tgggtgggct tgggggcagg tggggccaca gcctgaggag ggcctggggc
      541 taggactggg acaggggctg gggcctgggc ca
//
