LOCUS       sequence-60234-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Lamothe B, Besse A, Campos AD, Webster WK, Wu H, Darnay BG
  TITLE     Site-specific Lys-63-linked tumor necrosis factor 
            receptor-associated factor 6 auto-ubiquitination is a critical 
            determinant of I kappa B kinase activation.
  JOURNAL   J Biol Chem. 2007 Feb 9;282(6):4102-12. Epub 2006 Nov 29.
  PUBMED    17135271
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2007-02-9"; volume: "282(6)"; pages: "4102-12. Epub 
            2006 Nov 29"
COMMENT     Sequence Label: pCR3-FLAG-TRAF6-C70A
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             120..143
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             153..176
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttggt accgagctcg gatccactag taacggccgc cagtgtgctg gaattgatgg
      121 actacaagga cgacgatgac aaggaattga tggactacaa ggacgacgat gacaaggaat
      181 tcatgagtct cttaaactgt gagaacagct gcgggtccag ccagtcgtcc agtgactgct
      241 gcgctgccat ggccgcctcc tgcagcgctg cagtgaaaga tgacagcgtg agtggctctg
      301 ccagcaccgg gaacctctcc agctccttca tggaggagat ccagggctac gatgtggagt
      361 ttgacccacc tctggagagc aagtatgagg ctcccatatg cttgatggct ttacgggaag
      421 cagtgcaaac accatgtggc cacaggttct gcaaagcctg catcatcaaa tccataaggg
      481 atgcagggca caagtgccca gttgacaatg aaatactgct ggaaaatcaa ctgtttcccg
      541 acaattttgc aaagcgagag attctttccc tgacggtaaa gtgcccaaat aaaggctgtt
      601 tgcaaaagat ggaactgaga catctcgagg atcatcaagt acattgtgaa tttgctctag
      661 tgaattgtcc ccagtgccaa cgtcctttcc agaagtgcca ggttaataca cacattattg
      721 aggattgtcc caggaggcag gtttcttgtg taaactgtgc tgtgtccatg gcatatgaag
      781 agaaagagat ccatgatcaa agctgtcctc tggcaaatat catctgtgaa tactgtg
//
