LOCUS       sequence-60259-e        8702 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8702)
  AUTHORS   Rose T, Schoenenberger P, Jezek K, Oertner TG
  TITLE     Developmental refinement of vesicle cycling at schaffer collateral 
            synapses.
  JOURNAL   Neuron. 2013 Mar 20;77(6):1109-21. doi: 
            10.1016/j.neuron.2013.01.021.
  PUBMED    23522046
REFERENCE   2  (bases 1 to 8702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2013.01"; journalName: "Neuron"; date: 
            "2013-03-20- 20"; volume: "77"; issue: "6"; pages: "1109-21"
COMMENT     Sequence Label: pMH4-Syn1-Ratio2XsypHy
FEATURES             Location/Qualifiers
     source          1..8702
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_signal     191..341
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     promoter        393..840
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      896..905
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1457..1466
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1463..2179
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="MGSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     regulatory      2219..2228
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2225..2941
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="MGSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             3365..3391
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      3395..3404
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3399..3408
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3401..4078
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MVASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFL"
     CDS             4121..4795
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQA
                     LKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHHL
                     FL"
     polyA_signal    4809..4943
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4846..4865)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4900..4919
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             6233..6424
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(6418..6440)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6526..6666
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6581..6600
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6681..6698)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6852..7440)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6932..6951)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7611..8402,8403..8471))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8234..8253
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8472..8576)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8644..8662
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcccttccag ctctctgccc cttttggatt gaagccaata tgataatgag ggggtggagt
       61 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt
      121 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg
      181 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag
      241 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga
      301 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tctctagcat cgaagatccc
      361 tcgactctag actgcagagg gccctgcgta tgagtgcaag tgggttttag gaccaggatg
      421 aggcggggtg ggggtgccta cctgacgacc gaccccgacc cactggacaa gcacccaacc
      481 cccattcccc aaattgcgca tcccctatca gagaggggga ggggaaacag gatgcggcga
      541 ggcgcgtgcg cactgccagc ttcagcaccg cggacagtgc cttcgccccc gcctggcggc
      601 gcgcgccacc gccgcctcag cactgaaggc gcgctgacgt cactcgccgg tcccccgcaa
      661 actccccttc ccggccacct tggtcgcgtc cgcgccgccg ccggcccagc cggaccgcac
      721 cacgcgaggc gcgagatagg ggggcacggg cgcgaccatc tgcgctgcgg cgccggcgac
      781 tcagcgctgc ctcagtctgc ggtgggcagc ggaggagtcg tgtcgtgcct gagagcgcag
      841 tcgagaattc aagctgctag gtttaaacgg gccctctaga ctcgagctca agcttgccac
      901 catggacgtg gtgaatcagc tggtggctgg gggtcagttc cgggtggtca aggagcccct
      961 tggcttcgtg aaggtgctgc agtgggtctt tgccatcttc gcctttgcta cgtgcggcag
     1021 ctacaccgga gagcttcggc tgagcgtgga gtgtgccaac aagacggaga gtgccctcaa
     1081 catcgaagtc gaatttgagt acccattcag gctgcaccaa gtgtactttg atgcaccctc
     1141 ctgcgttaaa gggggcacta ccaagatctt cctagttggt gactactcct cctcgggtga
     1201 attctttgtc accgtggctg tgtttgcctt cctctactcc atgggggccc tggccaccta
     1261 catcttcctg cagaacaagt accgagagaa caacaaaggg ccaatgatgg acttcctggc
     1321 cacagcagtg ttcgctttca tgtggctagt tagctcatcc gcctgggcca aaggcctgtc
     1381 cgatgtgaag atggccactg acccagagaa cattatcaag gagatgccta tgtgccgcca
     1441 gacagatatg gatctagcca ccatgggaag taaaggagaa gaacttttca ctggagttgt
     1501 cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga
     1561 gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa
     1621 actacctgtt ccttggccaa cacttgtcac tactttaact tatggtgttc aatgcttttc
     1681 aagataccca gatcatatga aacggcatga ctttttcaag agtgccatgc ccgaaggtta
     1741 tgtacaggaa agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt
     1801 caagtttgaa ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga
     1861 agatggaaac attcttggac acaaattgga atacaactat aacgatcacc aggtgtacat
     1921 catggcagac aaacaaaaga atggaatcaa agctaacttc aaaattagac acaacattga
     1981 agatggaggc gttcaactag cagaccatta tcaacaaaat actccaattg gcgatgggcc
     2041 cgtcctttta ccagacaacc attacctgtt tacaacttct actctttcga aagatcccaa
     2101 cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg
     2161 catggatgaa ctatacaaaa ccggtggagc tcgaatctgc agaattcgcc cttggctagc
     2221 caccatggga agtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
     2281 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac
     2341 atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg ttccttggcc
     2401 aacacttgtc actactttaa cttatggtgt tcaatgcttt tcaagatacc cagatcatat
     2461 gaaacggcat gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat
     2521 atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac
     2581 ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg
     2641 acacaaattg gaatacaact ataacgatca ccaggtgtac atcatggcag acaaacaaaa
     2701 gaatggaatc aaagctaact tcaaaattag acacaacatt gaagatggag gcgttcaact
     2761 agcagaccat tatcaacaaa atactccaat tggcgatggg cccgtccttt taccagacaa
     2821 ccattacctg tttacaactt ctactctttc gaaagatccc aacgaaaaga gagaccacat
     2881 ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa
     2941 aaccggtgga gctcgaatca catgcaagga actgagggac cctgtgactt caggactcaa
     3001 cacctcggtg gtgtttggct tcctgaacct ggtgctctgg gttggcaacc tatggttcgt
     3061 gttcaaggag acaggctggg ccgccccatt catgcgcgca cctccaggcg ccccagaaaa
     3121 gcaaccagct cctggcgatg cctacggcga tgcgggctat gggcagggcc ccggaggcta
     3181 tgggccccag gactcctacg ggcctcaggg tggttatcaa cccgattacg ggcagccagc
     3241 cagcggcggt ggcggtggct acgggcctca gggcgactat gggcagcaag gctacggcca
     3301 acagggtgcg cccacctcct tctccaatca gatgtcggat ccgaagcttc ggtaccaagc
     3361 tgcctaccct tacgatgtgc ccgattacgc cgcggccgcc atggtggcct cctccgagga
     3421 cgtcatcaaa gagttcatgc gcttcaaggt gcgcatggag ggctccgtga acggccacga
     3481 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
     3541 gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcca
     3601 gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc cccgactaca agaagctgtc
     3661 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac
     3721 cgtgacccag gactcctccc tgcaggacgg cacgctgatc tacaaggtga agttccgcgg
     3781 caccaacttc ccccccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
     3841 caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc gagatccacc aggccctgaa
     3901 gctgaaggac ggcggccact acctggtgga gttcaagacc atctacatgg ccaagaagcc
     3961 cgtgcagctg cccggctact actacgtgga caccaagctg gacatcacct cccacaacga
     4021 ggactacacc atcgtggaac agtacgagcg ctccgagggc cgccaccacc tgttcctggg
     4081 gcatggcacc ggcagcaccg gcagcggcag ctccggcacc gcctcctccg aggacgtcat
     4141 caaagagttc atgcgcttca aggtgcgcat ggagggctcc gtgaacggcc acgagttcga
     4201 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
     4261 gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt tccagtacgg
     4321 ctccaaggcg tacgtgaagc accccgccga catccccgac tacaagaagc tgtccttccc
     4381 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
     4441 ccaggactcc tccctgcagg acggcacgct gatctacaag gtgaagttcc gcggcaccaa
     4501 cttccccccc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga
     4561 gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa
     4621 ggacggcggc cactacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca
     4681 gctgcccggc tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta
     4741 caccatcgtg gaacagtacg agcgctccga gggccgccac cacctgttcc tgtaggtcga
     4801 cttcgagcaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     4861 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     4921 atgtatctta tcatgtctgg atcgtctagc atcgaagatc cctgtcctcg accgatgccc
     4981 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc
     5041 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg
     5101 gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg
     5161 gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt
     5221 ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg
     5281 ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc
     5341 ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag
     5401 ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcac tggaccgctg
     5461 atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc atggattgta
     5521 ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc
     5581 acctcgacct gaatggaagc cggcggcacc tcgctaacgg attcaccact ccaagaattg
     5641 gagccaatca attcttgcgg agaactgtga atgcgcaaac caacccttgg cagaacatat
     5701 ccatcgcgtc cgccatctcc agcagccgca cgcggcgcat ctcgggcagc gttgggtcct
     5761 ggccacgggt gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc tggcggggtt
     5821 gccttactgg ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc gactgctgct
     5881 gcaaaacgtc tgcgacctga gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa
     5941 gtctggaaac gcggaagtca gcgccctgca ccattatgtt ccggatctgc atcgcaggat
     6001 gctgctggct accctgtgga acacctacat ctgtattaac gaagcgctgg cattgaccct
     6061 gagtgatttt tctctggtcc cgccgcatcc ataccgccag ttgtttaccc tcacaacgtt
     6121 ccagtaaccg ggcatgttca tcatcagtaa cccgtatcgt gagcatcctc tctcgtttca
     6181 tcggtatcat tacccccatg aacagaaatt cccccttaca cggaggcatc aagtgaccaa
     6241 acaggaaaaa accgccctta acatggcccg ctttatcaga agccagacat taacgcttct
     6301 ggagaaactc aacgagctgg acgcggatga acaggcagac atctgtgaat cgcttcacga
     6361 ccacgctgat gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg gtgaaaacct
     6421 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     6481 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca
     6541 gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta
     6601 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     6661 atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     6721 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     6781 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     6841 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     6901 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     6961 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     7021 ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag
     7081 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     7141 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     7201 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     7261 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     7321 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     7381 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     7441 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     7501 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     7561 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     7621 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     7681 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     7741 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     7801 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     7861 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     7921 attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     7981 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     8041 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     8101 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     8161 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     8221 gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     8281 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     8341 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     8401 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     8461 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     8521 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     8581 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     8641 aaaaataggc gtatcacgag gccctttcgt cttcaagaat tgatccgggc ccccatttcc
     8701 cc
//
