LOCUS       sequence-60263-e         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Lamothe B, Besse A, Campos AD, Webster WK, Wu H, Darnay BG
  TITLE     Site-specific Lys-63-linked tumor necrosis factor 
            receptor-associated factor 6 auto-ubiquitination is a critical 
            determinant of I kappa B kinase activation.
  JOURNAL   J Biol Chem. 2007 Feb 9;282(6):4102-12. Epub 2006 Nov 29.
  PUBMED    17135271
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2007-02-9"; volume: "282(6)"; pages: "4102-12. Epub 
            2006 Nov 29"
COMMENT     Sequence Label: pCR3-FLAG-Ubc13-WT
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             120..143
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             153..176
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(654..672)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(655..672)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(692..709)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    698..809
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttggt accgagctcg gatccactag taacggccgc cagtgtgctg gaattgatgg
      121 actacaagga cgacgatgac aaggaattga tggactacaa ggacgacgat gacaaggaat
      181 tcgccgggct gccccgcagg atcatcaagg aaacccagcg tttgctggca gaaccagttc
      241 ctggcatcaa agccgaacca gatgagagca acgcccgtta ttttcatgtg gtcattgctg
      301 gccctcagga ttcccccttt gagggaggga cttttaaact tgaactattc cttccagaag
      361 aatacccaat ggcagcccct aaagtacgtt tcatgaccaa aatttatcat cctaatgtag
      421 acaagttggg aagaatatgt ttagatattt tgaaagataa gtggtcccca gcactgcaga
      481 tccgcacagt tctgctatcg atccaggcct tgttaagtgc tcccaatcca gatgatccat
      541 tagcaaatga tgtagcggag cagtggaaga ccaacgaagc ccaagccata gaaacagcta
      601 gagcatggac taggctatat gccatgaata atatttaatc tagagggccc tattctatag
      661 tgtcacctaa atgctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca
      721 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
      781 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggt
//
