LOCUS       sequence-60332-e        7459 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7459)
  AUTHORS   Pijnappel WW, Esch D, Baltissen MP, Wu G, Mischerikow N, Bergsma AJ,
            van der Wal E, Han DW, Bruch Hv, Moritz S, Lijnzaad P, Altelaar AF, 
            Sameith K, Zaehres H, Heck AJ, Holstege FC, Scholer HR, Timmers HT
  TITLE     A central role for TFIID in the pluripotent transcription circuitry.
  JOURNAL   Nature. 2013 Mar 28;495(7442):516-9. doi: 10.1038/nature11970. Epub 
            2013 Mar 17.
  PUBMED    23503660
REFERENCE   2  (bases 1 to 7459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature11970"; journalName: "Nature"; date: "2013-03-28- 
            28"; volume: "495"; issue: "7442"; pages: "516-9"
COMMENT     Sequence Label: pMX_mTAF3
FEATURES             Location/Qualifiers
     source          1..7459
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1094..1510
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1440..1462
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1478..1494
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1520..1894
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1832..1848
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             1951..1974
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     LTR             4964..5433
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5563..5580)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5734..6322)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5814..5833)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6486..7277,7278..7346))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7109..7128
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7347..7451)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
     1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
     1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
     1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
     1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg
     1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac
     1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc
     1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaaggat
     1921 cccagtgtgg tggtacggga attcaccatg gattataaag atgatgatga taaatgcgag
     1981 agttactcca ggtcgttgtt gagggtctcg gtggcgcaga tctgccaggc gctgggctgg
     2041 gactcggtgc agctcagcgc ctgccacctc ctcaccgacg tcctgcagcg ctatctgcag
     2101 cagctgggcc ggggctgcca tcggtactct gaactctatg gccgaacaga ccccattttg
     2161 gatgatgttg gtgaagcctt ccaacttatg ggagttaatc tgcatgaact agaagactat
     2221 attcataata ttgaacctgt cactttccca catcaaattc cttcatttcc tgttagcaag
     2281 aacaatgtcc ttcagtttcc tcagcccgga agtaaagatg cagaggaaag aaaagactac
     2341 attccggatt accttccccc catcgtgtct tctcaagaag aggaggaaga ggaacaggtg
     2401 cccactgatg ggggcacctc agcagaagcc atgcaggtgc ccttggaaga agatgatgag
     2461 atggaggagg aggaagtcat caatgatgag aacttcttgg gtaagaggcc actggacagt
     2521 cctgaagtgg aagaaatgcc ctccatgaag cgcccacgac tcctgagcac taaaggggac
     2581 tccctagatg ttgtgttgtt agaagcacga gaacccctca gctctataaa cccacagaag
     2641 accccaccag tgctctctcc tgtgcgtgtc caggacaggg ctgatctggc ccctccatca
     2701 ccacagcctc ccatgctggc tccatttgca aagtcacaac taccaattgc aaagccgtta
     2761 gaaacaaagt cgtttacacc aaaaacaaag actaaagcta gctctccagg tcagaaaact
     2821 aagtcaccca aggctgccct atcaccagca aggcttggaa gtcctattcg gtcaccaaaa
     2881 accatcccaa aagaaaagaa gtcacctgga cgctccaaga gccccaagag tcctaagagc
     2941 cccaagattg tagctcatgt tccccaaacc cctgtcagac ctgaaacacc aaataggacc
     3001 ccttcagcca tggtggttga aaaaactgtt aaagagacca tcccagtgat gaaaccaaca
     3061 cagactcccc cagaagttgt gaaactgaac attgaaatgc agccgaaaaa gcctgtggtg
     3121 acagacaaaa ccatcgatga ctccatcgat gctgtgatcg cacgtgcctg tgcagagcgg
     3181 gagccagacc ctttcgagtt ttcttccgga tcggaatcgg aaggagacac ttttacaagt
     3241 cctaagagga tttcaggctc agagtgcgcc actccaaaag cctccacttc ctccaataac
     3301 ttcaccaagt ccctggccac tcctctgcct ctctccagcg gaacctcaag ttcagataac
     3361 tcgtggacaa tggatgcctc tattgatgag gtggtacgga aggcaaagct gggagcccct
     3421 tccaacatgc ctcccacctt cccatatatc tcttctccct caatttctcc tcccactcct
     3481 gagcctctac acaaggggta tgaggagaaa gccaagctgc cttcgtctgt ggatgtaaag
     3541 aaaaagttga aaaaagaact gaagactaag ttgaaaaaga aagaaaagca gcgagacaga
     3601 gagagggaga gagagagaaa caaagaaaga agcaaagaga aagataaaat gagagagagg
     3661 gagaaggaga aggaggctgg aaaggaactt aagtacccgt ggagggagct gatgaaagac
     3721 gaagattctg atccctataa gtttaaaatc aaagaatttg aagacattga tgctgcgaaa
     3781 gtgcgattga aagatgggat cgtgaggaga gagcgagaga aacataagga caagaagaaa
     3841 gatcgagaaa gaagcaagcg ggagaaagat aagcgagaaa gggaaaggct taaagaaaaa
     3901 aacagagaag acaagataaa ggcccctcca acacagctgg tgttgccccc aaaagaaatg
     3961 gcactgcctt tgttcagccc ttcagcagtg agggtcccgg ccatgctgcc ggccttctca
     4021 ccaatgctcc cagaaaaact gtttgaggaa aaagagaagc ccaaggagaa agaaaggaaa
     4081 aaggacaaaa aggagaagaa gaagaagaaa gagaaggaga aagaaaagga gaagaaagag
     4141 agggagaggg agaaggagag aagggagaga gagaagagag agaaggagaa ggagaagcac
     4201 aaacatgaga agataaaagt ggagcctgtc atccctgccc caagtccagt tatccccagg
     4261 ttgactctgc gagttggcgc tggccaggac aagattgtta tcagcaaggt ggtaccagcc
     4321 ccggaggcta agcccgctcc ttctctgaac agacccaaaa ccccacctcc agccccagtc
     4381 cctattcctg tgcgagtgag ccctactccc ctgcagccac cactcctcac ccaggctgca
     4441 gtgtgcccag ccctgatgcc atccccagct cccgccctct ctggaatagg gagtgccaaa
     4501 gcccccgtga ggagcgtcgt gactgagacg gtcagcactt atgtgatccg tgatgagtgg
     4561 ggcaatcaga tctggatctg tccagggtgc aacaagcctg atgacgggag cccgatgatt
     4621 ggctgtgacg actgtgacga ttggtaccac tggccctgtg tgggaatcat ggctgctcct
     4681 ccagaagaga tgcagtggtt ctgcccgaag tgtgccaaca agataaagaa agacaagaag
     4741 cacaagaaga ggaagcaccg tgcgcactga ctcgagcggc cgccagcaca gtggtcgacg
     4801 ataaaataaa agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt
     4861 aggtttggca agctagctta agtaacgcca ttttgcaagg catggaaaaa tacataactg
     4921 agaatagaga agttcagatc aaggtcagga acagatggaa cagctgaata tgggccaaac
     4981 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggaacagct
     5041 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
     5101 acagatggtc cccagatgcg gtccagccct cagcagtttc tagagaacca tcagatgttt
     5161 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
     5221 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
     5281 ctcactcggg gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata
     5341 aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg
     5401 agtgattgac tacccgtcag cgggggtctt tcacatgcag catgtatcaa aattaatttg
     5461 gttttttttc ttaagtattt acattaaatg gccatagttg cattaatgaa tcggccaacg
     5521 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     5581 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     5641 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     5701 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     5761 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     5821 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     5881 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     5941 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     6001 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     6061 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     6121 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     6181 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     6241 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     6301 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     6361 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     6421 ctagatcctt ttgcggccgg ccgcaaatca atctaaagta tatatgagta aacttggtct
     6481 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     6541 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     6601 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     6661 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     6721 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     6781 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     6841 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     6901 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     6961 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     7021 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     7081 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     7141 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     7201 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     7261 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     7321 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     7381 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     7441 ggggttccgc gcacatttc
//
