LOCUS       sequence-60334-e        7010 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7010)
  AUTHORS   Pijnappel WW, Esch D, Baltissen MP, Wu G, Mischerikow N, Bergsma AJ,
            van der Wal E, Han DW, Bruch Hv, Moritz S, Lijnzaad P, Altelaar AF, 
            Sameith K, Zaehres H, Heck AJ, Holstege FC, Scholer HR, Timmers HT
  TITLE     A central role for TFIID in the pluripotent transcription circuitry.
  JOURNAL   Nature. 2013 Mar 28;495(7442):516-9. doi: 10.1038/nature11970. Epub 
            2013 Mar 17.
  PUBMED    23503660
REFERENCE   2  (bases 1 to 7010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature11970"; journalName: "Nature"; date: "2013-03-28- 
            28"; volume: "495"; issue: "7442"; pages: "516-9"
COMMENT     Sequence Label: pMX_hTAF5
FEATURES             Location/Qualifiers
     source          1..7010
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1094..1510
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1440..1462
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1478..1494
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1520..1894
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1832..1848
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     LTR             4515..4984
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5114..5131)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5285..5873)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5365..5384)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6037..6828,6829..6897))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6660..6679
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6898..7002)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
     1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
     1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
     1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
     1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg
     1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac
     1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc
     1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaatggc
     1921 tcagccgcaa gatggcggcg ctggcggagg agcagacgga ggtggcggtc aagctagagc
     1981 ctgagggacc gccaacgctg ctacctccgc aggcggggga cggcgcaggc gagggtagcg
     2041 gcggcactac caacaacggc cccaacggcg gcggcgggaa cgttgcggcg tcgtcgtcca
     2101 ctggcgggga tggcgggacc cccaagccca cggtggctgt ctccgccgct gccccggcgg
     2161 gggcggcccc ggtgcccgcc gctgctccgg acgccggcgc tccgcatgac cgacagactc
     2221 tactggccgt gctgcagttc ctacggcaga gcaaactccg cgaggccgaa gaggcgctgc
     2281 gccgtgaggc cgggctgctg gaggaggcag tggcgggctc cggagccccg ggagaggtgg
     2341 acagcgccgg cgctgaggtg accagcgcgc ttctcagccg ggtgaccgcc tcggcccctg
     2401 gccctgcggc ccccgaccct ccgggcactg gcgcttcggg ggccacggtc gtctcaggtt
     2461 cagcctcagg tcctgcggct ccgggtaaag ttggaagtgt tgctgtggaa gaccagccag
     2521 atgtcagtgc cgtgttgtca gcctacaacc aacaaggaga tcccacaatg tatgaagaat
     2581 actatagtgg actgaaacac ttcattgaat gttccctgga ctgccatcgg gcagagttgt
     2641 cccaactttt ttatcctctg tttgtgcaca tgtacttgga gctagtctac aatcaacatg
     2701 agaatgaagc aaagtcattc tttgagaagt tccatggaga tcaggaatgt tattaccagg
     2761 atgacctacg agtattatct agtcttacca aaaaggaaca catgaaaggg aatgagacca
     2821 tgttggattt tcgaacaagt aaatttgttc tgcgtatttc ccgtgactcg taccaactct
     2881 tgaagaggca tcttcaggag aaacagaaca atcagatatg gaacatagtt caggagcacc
     2941 tctacattga catctttgat gggatgccgc gtagtaagca acagatagat gcgatggtgg
     3001 gaagtttggc aggagaggct aaacgagagg caaacaaatc aaaggtattt tttggtttat
     3061 taaaagaacc agaaattgag gtacctttgg atgacgagga tgaagaggga gaaaatgaag
     3121 aaggaaaacc taaaaagaag aagcctaaaa aagatagtat tggatccaaa agcaaaaaac
     3181 aagatcccaa tgctccacct cagaacagaa tccctcttcc tgagttgaaa gattcagata
     3241 agttggataa gataatgaat atgaaagaaa ccaccaaacg agtgcgcctt gggccggact
     3301 gcttaccctc catttgtttc tatacatttc tcaatgctta ccagggtctc actgcagtgg
     3361 atgtcactga tgattctagt ctgattgctg gaggttttgc agattcaact gtcagagtgt
     3421 ggtcggtaac acccaaaaag cttcgtagtg tcaaacaagc atcagatctt agtcttatag
     3481 acaaagaatc agatgatgtc ttagaaagaa tcatggatga gaaaacagca agtgagttga
     3541 agattttgta tggtcacagt gggcctgtct acggagccag cttcagtccg gataggaact
     3601 atctgctttc ctcttcagag gacggaactg ttagattgtg gagccttcaa acatttactt
     3661 gtttggtggg atataaagga cacaactatc cagtatggga cacacaattt tctccatatg
     3721 gatattattt tgtgtcaggg ggccatgacc gagtagctcg gctctgggct acagaccact
     3781 atcagccttt aagaatattt gccggccatc ttgctgatgt gaattgtacc agattccatc
     3841 caaattctaa ttatgttgct acgggctctg cagacagaac tgtgcggctc tgggacgtcc
     3901 tgaatggtaa ctgtgtaagg atcttcactg gacacaaggg accaattcat tccttgacat
     3961 tttctcccaa tgggagattc ctggctacag gagcaacaga tggcagagtg cttctttggg
     4021 atattggaca tggtttgatg gttggagaat taaaaggcca cactgataca gtctgttcac
     4081 ttaggtttag tagagatggt gaaattttgg catcaggttc aatggataat acagttcgat
     4141 tatgggatgc tatcaaagcc tttgaagatt tagagaccga tgactttact acagccactg
     4201 ggcatataaa tttacctgag aattcacagg agttattgtt gggaacatat atgaccaaat
     4261 caacaccagt tgtacacctt cattttactc gaagaaacct ggttctagct gcaggagctt
     4321 atagtccaca ataaaccatc ggtagtcgac gataaaataa aagattttat ttagtctcca
     4381 gaaaaagggg ggaatgaaag accccacctg taggtttggc aagctagctt aagtaacgcc
     4441 attttgcaag gcatggaaaa atacataact gagaatagag aagttcagat caaggtcagg
     4501 aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc
     4561 cggctcaggg ccaagaacag atggaacagc tgaatatggg ccaaacagga tatctgtggt
     4621 aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc ggtccagccc
     4681 tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc
     4741 ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc
     4801 tccccgagct caataaaaga gcccacaacc cctcactcgg ggcgccagtc ctccgattga
     4861 ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg
     4921 gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct
     4981 ttcacatgca gcatgtatca aaattaattt ggtttttttt cttaagtatt tacattaaat
     5041 ggccatagtt gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     5101 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     5161 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     5221 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     5281 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     5341 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     5401 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     5461 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     5521 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     5581 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     5641 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     5701 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     5761 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     5821 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     5881 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     5941 tggtcatgag attatcaaaa aggatcttca cctagatcct tttgcggccg gccgcaaatc
     6001 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     6061 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     6121 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     6181 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     6241 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     6301 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     6361 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     6421 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     6481 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     6541 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     6601 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     6661 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     6721 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     6781 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     6841 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     6901 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     6961 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
//
