LOCUS       sequence-60335-e        6807 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6807)
  AUTHORS   Pijnappel WW, Esch D, Baltissen MP, Wu G, Mischerikow N, Bergsma AJ,
            van der Wal E, Han DW, Bruch Hv, Moritz S, Lijnzaad P, Altelaar AF, 
            Sameith K, Zaehres H, Heck AJ, Holstege FC, Scholer HR, Timmers HT
  TITLE     A central role for TFIID in the pluripotent transcription circuitry.
  JOURNAL   Nature. 2013 Mar 28;495(7442):516-9. doi: 10.1038/nature11970. Epub 
            2013 Mar 17.
  PUBMED    23503660
REFERENCE   2  (bases 1 to 6807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature11970"; journalName: "Nature"; date: "2013-03-28- 
            28"; volume: "495"; issue: "7442"; pages: "516-9"
COMMENT     Sequence Label: pMX-mTAF6
FEATURES             Location/Qualifiers
     source          1..6807
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1094..1510
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1440..1462
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1478..1494
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1520..1894
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1832..1848
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     LTR             4312..4781
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4911..4928)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5082..5670)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5162..5181)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5834..6625,6626..6694))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6457..6476
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6695..6799)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
     1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
     1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
     1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
     1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg
     1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac
     1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc
     1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaaggat
     1921 cccagtgtgg tggtacggga atttccagca tggccgagga gaagaaactg aaactcagca
     1981 atactgtgtt gccctctgag tccatgaagg tggtggcaga atctatgggc attgctcaga
     2041 ttcaggagga gacttgccag ctactgacag atgaggtcag ctaccgcatc aaggagattg
     2101 cccaggatgc cctgaagttc atgcacatgg ggaaacgaca gaagctcact accagtgaca
     2161 ttgactatgc attgaagtta aagaatgtgg agccactgta tggcttccat gctcaggagt
     2221 tcatcccttt ccggtttgcc tctggcgggg gccgggagct gtacttctat gaagagaagg
     2281 aagtggacct gagtgacatc atcaacaccc ctctgcctcg ggtgcctctg gatgtctgcc
     2341 tcaaagctca ttggctaagc atcgaaggct gccagccagc tataccagag aatccacccc
     2401 cagctcccaa agagcagcag aaggctgagg ccacagaacc cctgaaatca gcaaagccag
     2461 gccaagaaga agatggaccc ctgaaaggca aaggtcaagg ggcagctgca gctgatggca
     2521 aaggaaaaga gaagaaggca cctcccttgc tggagggggc ccctttccgc ctgaagcccc
     2581 gcagcatcca tgagctgtct gtggagcagc agctgtatta caaggagatc acggaagcct
     2641 gtgtgggatc ctgtgaggcc aagagagcgg aagctctgca gagcattgcc acggacccag
     2701 ggctctatca gatgctacca cggttcagta ccttcatctc agagggggtc cgtgtgaatg
     2761 tagttcagaa taacctggcc ttgctcatct acctgatgcg tatggtgaag gccctgatgg
     2821 acaaccccac actgtatcta gagaaatatg tgcatgagct cattccagca gtaatgacct
     2881 gcattgtgag tagacagctg tgcttgcggc cagatgtgga caatcactgg gcactccggg
     2941 attttgctgc ccgtctagtg gcccaaatct gtaagcattt cagcacaacc accaacaaca
     3001 tccaatcccg aatcactaag acctttacca agagctgggt cgacgagaag actccatgga
     3061 caactcgata tggctccatt gcaggcctag cagagctggg acatgatgtg attaagacgc
     3121 taattctgcc aaggctgcag caggaagggg aacggatccg cagtgtcttg gatgggcctg
     3181 tgctgagcaa catcgaccgt attggagcag accatgtgca gagccttctc ctgaaacact
     3241 gtgccccagt actggccaag ctgcgccctc cacctgacaa tcaggatgcc taccggggag
     3301 aatttgggtc ccttgggccc cttctctgtt cccatgtggt gaaggccagg gcccaggctg
     3361 ctctacaagc tcagcaggtc aacaggacca cactcaccat cacacagcct cggcccacac
     3421 tgaccctttc acaggcccct cagccaggcc ctcgcacgcc tggcttattg aaggtccctg
     3481 gctccattgc cttgcctgtt cagacactgg tgtctgcgag agctgctgct cctcctcagc
     3541 cttctcctcc tccaaccaag tttattgtaa tgtcctcatc ttccagtgcc tcatcaaccc
     3601 agcaggttct gtccctcagc acctcagctc ctggctcagg ctctactact acctctcctg
     3661 tcaccaccac ggttcccagc gtgcagccca tcgtcaagct ggtctccact gctaccactg
     3721 cacccccaag cacagcccct gctggttctg gcagtgttca gaaatacatt gtggtctctc
     3781 ttcccccaac aggggaaggc aaaggaggcc ctccttctca tccttctcca gtcccccctt
     3841 catcttcttc accatcccca cttgggggaa gtaccctctg tggggggaag caggaagctg
     3901 gggacagccc ccctccagct ccagggactc caaaggctaa tggctcgcag cctactggac
     3961 ctggctctcc ccagcctgct ctgtgatgtg tcacatgaat tccagctgag cgccggtcgc
     4021 taccattacc agttggtctg gtgtcaaaaa taataataac cgggcaggcc atgtctgccc
     4081 gtatttcgcg taaggaaatc cattatgtac tatttaaact cgagcggccg ccagcacagt
     4141 ggtcgacgat aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc
     4201 ccacctgtag gtttggcaag ctagcttaag taacgccatt ttgcaaggca tggaaaaata
     4261 cataactgag aatagagaag ttcagatcaa ggtcaggaac agatggaaca gctgaatatg
     4321 ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg
     4381 gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag
     4441 ggccaagaac agatggtccc cagatgcggt ccagccctca gcagtttcta gagaaccatc
     4501 agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca
     4561 atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc
     4621 cacaacccct cactcggggc gccagtcctc cgattgactg agtcgcccgg gtacccgtgt
     4681 atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt
     4741 ctcctctgag tgattgacta cccgtcagcg ggggtctttc acatgcagca tgtatcaaaa
     4801 ttaatttggt tttttttctt aagtatttac attaaatggc catagttgca ttaatgaatc
     4861 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     4921 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     4981 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     5041 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     5101 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     5161 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     5221 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     5281 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     5341 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     5401 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     5461 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     5521 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     5581 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     5641 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     5701 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     5761 atcttcacct agatcctttt gcggccggcc gcaaatcaat ctaaagtata tatgagtaaa
     5821 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     5881 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     5941 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     6001 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     6061 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     6121 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     6181 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     6241 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     6301 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     6361 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     6421 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     6481 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     6541 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     6601 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     6661 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     6721 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     6781 aacaaatagg ggttccgcgc acatttc
//
