LOCUS       sequence-60342-e        7544 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7544)
  AUTHORS   Pijnappel WW, Esch D, Baltissen MP, Wu G, Mischerikow N, Bergsma AJ,
            van der Wal E, Han DW, Bruch Hv, Moritz S, Lijnzaad P, Altelaar AF, 
            Sameith K, Zaehres H, Heck AJ, Holstege FC, Scholer HR, Timmers HT
  TITLE     A central role for TFIID in the pluripotent transcription circuitry.
  JOURNAL   Nature. 2013 Mar 28;495(7442):516-9. doi: 10.1038/nature11970. Epub 
            2013 Mar 17.
  PUBMED    23503660
REFERENCE   2  (bases 1 to 7544)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature11970"; journalName: "Nature"; date: "2013-03-28- 
            28"; volume: "495"; issue: "7442"; pages: "516-9"
COMMENT     Sequence Label: pMX-mTAF4b
FEATURES             Location/Qualifiers
     source          1..7544
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1094..1510
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1440..1462
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1478..1494
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1520..1894
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1832..1848
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             2077..2142
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2161..2274
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     LTR             5049..5518
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5648..5665)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5819..6407)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5899..5918)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6571..7362,7363..7431))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7194..7213
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7432..7536)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
     1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
     1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
     1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
     1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg
     1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac
     1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc
     1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaaggat
     1921 cccagtgtgg tggtacggga attccagctg agcgccggtc gctaccatta ccagttggtc
     1981 tggtgtcaaa aataataata accgggcagg ccatgtctgc ccgtatttcg cgtaaggaaa
     2041 tccattatgt actatttaaa ctcgagcggc cgcatggact acaaagacca tgacggtgat
     2101 tataaagatc atgacatcga ctacaaggat gacgatgaca agggaagcgg tagcggtacc
     2161 atggacgaga agaccaccgg ctggcggggc ggccacgtgg tggagggcct ggccggcgag
     2221 ctggagcagc tgcgggccag gctggagcac caccctcagg gccagcggga gccctccggc
     2281 ggctgcaagc tgggcgcccg ggcggatccc atgccggcgg gcctcacgga gccggccggc
     2341 gccgctgctc ctgtagtctc gagcgcgtcg ggggccgtga ccatggctcc ggtcgccgcg
     2401 ctgcccgtcc gggtggaggg caccccggtg gccctgggcc ctgtgactaa ggctcctgtc
     2461 agtgtttgcg tggagtctgt ggcgccccag cccttgccag ccccagtggg gactctagtg
     2521 accaaagtgg ttcctgtcac tgcccttcct aaactcggca gccctaggct gcccgctcct
     2581 cagatagtca ccgtgaaaac gccgggcacc acgacaatcc aactaccggc taatttgcag
     2641 cttcctccag gaacagtttt gatcaaaagt aacagtggtc agttgatgct ggtatcgcct
     2701 cagcaagctg taacaggagc caagaccaca agtaacataa ctccaagacc agcagtacca
     2761 gcaaacactc aaacagtaaa gatctgtaca gtgccgaatt ctagctcaca attaatgaag
     2821 aaagtaacag tggcgcctgt taaaaatttg acacagatag gaactactgt ggcaaccact
     2881 gcctccagta cttcctcagg acaacctgtg gctctaccaa gcagtgtcat cacagttact
     2941 cctgcaaagc tggtgaatac tgtatccacc ctgaagtcat caagtttggg agtgttatct
     3001 accccctcaa atgacgcacg tctcaaagct gagacttcag tagctgctca gactgctctt
     3061 cctccgacag tgctagaaaa tgtgaaaaaa tgtaaaaact tcctttcaat gttaataaaa
     3121 ctagcatgta gtggatcaca gtctccagaa atggggcaga atgtgaagag gctggtggag
     3181 cagcttttgg atgcagaaat tgaagcagaa gagtttacta ggaaactgta tattgaactc
     3241 aagtctgcac ctcagccgca cctcgtccct tttcttaaga agagtgtggt tgccctaaga
     3301 cagcttcttc caaattccca gagcttcatt gagaactgtg tgaaggaagt ttctggtgat
     3361 gtggtcattt cctcctgtac catgacaaca gcaacgtctc ctgtggtgac aagtacagtt
     3421 tccccagtcc ttgtgtctgg agcaacagca cccagaactc tgtctgttca gcagaccctg
     3481 aatccacttg ctggtccagg agtagcaaac actggagttg tgacactgca ttctgttgcc
     3541 ccagctgctg caacaggagg tacgacagct gcaactgttt tgcttcaaac ttcaaaacca
     3601 ctaacaacat cagtaccaaa tacagtggca gcagtgtccc ttcagcctga gaatccagtt
     3661 gtgtctgggg cagcagtaac actggctatt ccatcagcaa cttttggaga agcgtccgct
     3721 acacctcttt gtcttccatc tgcgaagcct gccattacct ctgctgggac caaagctgat
     3781 aaacctgcca ttggcactcc agtccagatc gttacacaac cgagcaccct ccttccacag
     3841 gcggctggca ttccacagac agccaaagtc aagcaactag ttgtacagca gccttcagga
     3901 agcagtgtga atcatgtgac ctccatttcc cattcctcac cattgagcac tcagaactgt
     3961 gggcagaaga caccagtaaa tgctgtgatg cctactactt ccatcataaa gcaaattact
     4021 ctgcctggaa ataaactttt gtcgcttcaa gctcaaagat cttctattca gagcaataaa
     4081 ataaaagaga atggaccaac atgcttcaga ggtgaagatg acatcaatga tgtgactttc
     4141 atggcagagg tcaaccttga tgaagaaaat gcctgcatct tagcagcaca ttctgacttt
     4201 gttggcacac ttattcagtc atgtaaagaa gaaccattcc ttgtcattgg agctctacag
     4261 aagagaattt tagacattgg taaaaagcat gacattacag aacttaactc tgatgctgtg
     4321 aacttgatct cccatgcaac acaggagaga ttacgaggcc ttctagagaa actaactaca
     4381 attgctcagc atcgaatgac tatttacaag ggaagtgaaa attatatctt gtctactgat
     4441 accagatcac agctcaaatt tcttgaaaag ctcgatcagt tggagaaaca gaggaaggat
     4501 ttagaagaaa gagagatgtt actaaaggca gccaagagtc gctccaataa agaagatcca
     4561 gagcagctga gattaaaaca aaaagccaaa gagttgcagc agttggagct tgcccagatc
     4621 cagtacagag atgctaatct cacagctctt gcagctattg gaccaaggaa gaagagacca
     4681 ctagaatcag gaaatgagag cttcaaagat aacccttcta cttctgggac ttccagccta
     4741 acagccacca aaccatttcg tccaagaatc acaagaatct gcctcaggga tttgatattc
     4801 tgtatggaac aggaaagaga gatgaagtat tctcgagccc tataccttgc ccttcttaag
     4861 tgaaagctta tcgataccgt cgacgataaa ataaaagatt ttatttagtc tccagaaaaa
     4921 ggggggaatg aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg
     4981 caaggcatgg aaaaatacat aactgagaat agagaagttc agatcaaggt caggaacaga
     5041 tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc
     5101 agggccaaga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
     5161 ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtcca gccctcagca
     5221 gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc
     5281 cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg
     5341 agctcaataa aagagcccac aacccctcac tcggggcgcc agtcctccga ttgactgagt
     5401 cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg
     5461 ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcaca
     5521 tgcagcatgt atcaaaatta atttggtttt ttttcttaag tatttacatt aaatggccat
     5581 agttgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     5641 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     5701 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     5761 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     5821 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     5881 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     5941 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     6001 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     6061 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     6121 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     6181 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     6241 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     6301 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     6361 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     6421 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     6481 tgagattatc aaaaaggatc ttcacctaga tccttttgcg gccggccgca aatcaatcta
     6541 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     6601 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     6661 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     6721 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     6781 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     6841 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     6901 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     6961 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     7021 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     7081 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     7141 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     7201 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     7261 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     7321 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     7381 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     7441 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     7501 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttc
//
