LOCUS       sequence-60345-e         949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: I-GCCG
FEATURES             Location/Qualifiers
     source          1..949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(607..625)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(608..625)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(643..659)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(643..659)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(656..678)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    667..683
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(691..697,698..715,716..721))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    736..757
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(874..891)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctatagggcg aattgggccc tctagatgca tgctcgagcg gccgccagtg tgatggatat
       61 ctgcagaatt cgcccttgcc gtcctagcgc gtctcctgac cccagagcag gtcgtggcaa
      121 tcgcgagcaa taacggcgga aaacaggctt tggaaacggt gcagaggctc cttccagtgc
      181 tgtgccaagc gcacggactc accccagagc aggtcgtggc gatcgcaagc cacgacggag
      241 gaaagcaagc cttggaaaca gtacagaggc tgttgcctgt gctgtgccaa gcgcacggcc
      301 tcaccccaga gcaggtcgtg gcgatcgcaa gccacgacgg aggaaagcaa gccttggaaa
      361 cagtacagag gctgttgcct gtgctgtgcc aagcgcacgg attaacccca gagcaggtcg
      421 tggcaatcgc gagcaataac ggcggaaaac aggctttgga aacggtgcag aggctccttc
      481 cagtgctgtg ccaagcgcac ggcttaagag acgtgggcac aggaaagaag ggcgaattcc
      541 agcacactgg cggccgttac tagtggatcc gagctcggta ccaagcttga tgcatagctt
      601 gagtattcta tagtgtcacc taaatagctt ggcgtaatca tggtcatagc tgtttcctgt
      661 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
      721 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
      781 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
      841 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
      901 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacgg
//
