LOCUS       sequence-60349-e         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: I-GCAG
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(615..631)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(615..631)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(628..650)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    639..655
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(663..669,670..687,688..693))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    708..729
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(846..863)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctatagggcg aattgggccc tctagatgca tgctcgagcg gccgccagtg tgatggatat
       61 ctgcagaatt cgcccttata tagatgccgt cctagcgcgt ctcctgaccc cagagcaggt
      121 cgtggcaatc gcgagcaata acggcggaaa acaggctttg gaaacggtgc agaggctcct
      181 tccagtgctg tgccaagcgc acggactcac cccagagcag gtcgtggcga tcgcaagcca
      241 cgacggagga aagcaagcct tggaaacagt acagaggctg ttgcctgtgc tgtgccaagc
      301 gcacggcctc accccagagc aggtcgtggc aatcgcctcc aacattggcg ggaaacaggc
      361 actcgagact gtccagcgcc tgcttcccgt gctgtgccaa gcgcacggat taaccccaga
      421 gcaggtcgtg gcaatcgcga gcaataacgg cggaaaacag gctttggaaa cggtgcagag
      481 gctccttcca gtgctgtgcc aagcgcacgg cttaagagac gtgggcacag gaaagatact
      541 taagggcgaa ttccagcaca ctggcggccg ttactagtgg atccgagctc ggtaccaagc
      601 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
      661 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
      721 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
      781 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
      841 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      901 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      961 tgagcaaaag gccagc
//
