LOCUS       sequence-60357-e         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: I-GAGT
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(621..639)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(622..639)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(657..673)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(657..673)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(670..692)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    681..697
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(705..711,712..729,730..735))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    750..771
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(888..905)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctatagggcg aattgggccc tctagatgca tgctcgagcg gccgccagtg tgatggatat
       61 ctgcagaatt cgcccttata tagatgccgt cctagcgcgt ctcctgaccc cagagcaggt
      121 cgtggcaatc gcgagcaata acggcggaaa acaggctttg gaaacggtgc agaggctcct
      181 tccagtgctg tgccaagcgc acggactcac cccagagcag gtcgtggcaa tcgcctccaa
      241 cattggcggg aaacaggcac tcgagactgt ccagcgcctg cttcccgtgc tgtgccaagc
      301 gcacggcctc accccagagc aggtcgtggc aatcgcgagc aataacggcg gaaaacaggc
      361 tttggaaacg gtgcagaggc tccttccagt gctgtgccaa gcgcacggat taaccccaga
      421 gcaggtcgtg gccattgcct cgaatggagg gggcaaacag gcgttggaaa ccgtacaacg
      481 attgctgccg gtgctgtgcc aagcgcacgg cttaagagac gtgggcacag gaaagatact
      541 taagggcgaa ttccagcaca ctggcggccg ttactagtgg atccgagctc ggtaccaagc
      601 ttgatgcata gcttgagtat tctatagtgt cacctaaata gcttggcgta atcatggtca
      661 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
      721 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
      781 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
      841 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
      901 tcgctgcgct cggtcgttcg gctgcggcga g
//
