LOCUS       sequence-60371-e         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SPG7A
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             15..37
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             90..110
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 cctctagaaa taattttgtt taactttaag aaggagatat acatatgcac catcatcatc
       61 atcattcttc tggtgtagat ctgggtaccg agaacctgta cttccaatcc atgggagtca
      121 gcttcaaaga cgtggcagga atgcacgaag ccaaactgga agtccgcgag tttgtggatt
      181 atctgaagag cccagaacgc ttcctccagc ttggcgccaa ggtcccaaag ggcgcactgc
      241 tgctcggccc ccccggctgt gggaagacgc tgctggccaa ggcggtggcc acggaggctc
      301 aggtgccctt cctggcgatg gccggcgcag agttcgtgga ggtcattgga ggcctcggcg
      361 ctgcccgtgt gcggagcctc tttaaggaag cccgagcccg ggccccctgc atcgtctaca
      421 tcgatgagat cgacgcggtg ggcaagaagc gctccaccac catgtccggc ttctccaaca
      481 cggaggagga gcagacgctc aaccagcttc tggtagaaat ggatggaatg ggtaccacag
      541 accatgtcat cgtcctggcg tccacgaacc gagctgacat tttggacggt gctctgatga
      601 ggccaggccg actggaccgg cacgtcttca ttgatctccc cacgctgcag gagaggcggg
      661 agatttttga gcagcacctg aagagcctga agctgaccca gtccagcacc ttttactccc
      721 agcgtctggc agagctgaca ccaggattca gtggggctga catcgccaac atctgcaatg
      781 aggctgcgct gcacgcggcg cgggagggac acacttccgt gcacactctc aacttcgagt
      841 acgccgtgga gcgcgtcctc gcagggactg ccaaaaagag taagatcctg tccaagtgac
      901 agtaaaggtg gatacggatc cg
//
