LOCUS       sequence-60376-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: STARD5A
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             15..37
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             90..110
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             803..820
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(873..891)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 cctctagaaa taattttgtt taactttaag aaggagatat acatatgcac catcatcatc
       61 atcattcttc tggtgtagat ctgggtaccg agaacctgta cttccaatcc atggcagccc
      121 agatgagcga ggctgtggcc gagaagatgc tccagtaccg gcgggacaca gcaggctgga
      181 agatttgccg ggaaggcaat ggagtttcag tttcctggag gccatctgtg gagtttccag
      241 ggaacctgta ccgaggagaa ggcattgtat atgggacact agaggaggtg tgggactgtg
      301 tgaagccagc tgttggaggc ctacgagtga agtgggatga gaatgtgacc ggttttgaaa
      361 ttatccaaag catcactgac accctgtgtg taagcagaac ctccactccc tccgctgcca
      421 tgaagctcat ttctcccaga gattttgtgg acttggtgct agtcaagaga tatgaggatg
      481 ggaccatcag ttccaacgcc acccatgtgg agcatccgtt atgtcccccg aagccaggtt
      541 ttgtgagagg atttaaccat ccttgtggtt gcttctgtga acctcttcca ggggaaccca
      601 ccaagaccaa cctggtcaca ttcttccata ccgacctcag cggttacctc ccacagaacg
      661 tggtggactc cttcttcccc cgcagcatga cccggtttta tgccaacctt cagaaagcag
      721 tgaagcaatt ccatgagtga cagtaaaggt ggatacggat ccgaattcga gctccgtcga
      781 caagcttgcg gccgcactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa
      841 agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct
      901 tggggcctct aaacgggtct tgaggggttt
//
