LOCUS       sequence-60377-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MDA5A
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             15..37
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             90..110
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             821..838
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(891..909)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 cctctagaaa taattttgtt taactttaag aaggagatat acatatgcac catcatcatc
       61 atcattcttc tggtgtagat ctgggtaccg agaacctgta cttccaatcc atgaacagca
      121 acatgggcag tgattcaggc accatgggaa gtgattcaga tgaagagaat gtggcagcaa
      181 gagcatcccc ggagccagaa ctccagctca ggccttacca aatggaagtt gcccagccag
      241 ccttggaagg gaagaatatc atcatctgcc tccctacagg gagtggaaaa accagagtgg
      301 ctgtttacat tgccaaggat cacttagaca agaagaaaaa agcatctgag cctggaaaag
      361 ttatagttct tgtcaataag gtactgctag ttgaacagct cttccgcaag gagttccaac
      421 catttttgaa gaaatggtat cgtgttattg gattaagtgg tgatacccaa ctgaaaatat
      481 catttccaga agttgtcaag tcctgtgata ttattatcag tacagctcaa atccttgaaa
      541 actccctctt aaacttggaa aatggagaag atgctggtgt tcaattgtca gacttttccc
      601 tcattatcat tgatgaatgt catcacacca acaaagaagc agtgtataat aacatcatga
      661 ggcattattt gatgcagaag ttgaaaaaca atagactcaa gaaagaaaac aaaccagtga
      721 ttccccttcc tcagatactg ggactaacag cttcatgaca gtaaaggtgg atacggatcc
      781 gaattcgagc tccgtcgaca agcttgcggc cgcactcgag caccaccacc accaccactg
      841 agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
      901 ataactagca taaccccttg gggcctctaa
//
