LOCUS       sequence-60385-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: BCL2A1A
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             15..37
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             90..110
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             623..640
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(693..711)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      707..754
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(878..897)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cctctagaaa taattttgtt taactttaag aaggagatat acatatgcac catcatcatc
       61 atcattcttc tggtgtagat ctgggtaccg agaacctgta cttccaatcc atgacagact
      121 gtgaatttgg atatatttac aggctggctc aggactatct gcagtgcgtc ctacagatac
      181 cacaacctgg atcaggtcca agcaaaacgt ccagagtgct acaaaatgtt gcgttctcag
      241 tccaaaaaga agtggaaaag aatctgaagt catgcttgga caatgttaat gttgtgtccg
      301 tagacactgc cagaacacta ttcaaccaag tgatggaaaa ggagtttgaa gacggcatca
      361 ttaactgggg aagaattgta accatatttg catttgaagg tattctcatc aagaaacttc
      421 tacgacagca aattgccccg gatgtggata cctataagga gatttcatat tttgttgcgg
      481 agttcataat gaataacaca ggagaatgga taaggcaaaa cggaggctgg gaaaatggct
      541 ttgtaaagaa gtttgaatga cagtaaaggt ggatacggat ccgaattcga gctccgtcga
      601 caagcttgcg gccgcactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa
      661 agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct
      721 tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatt
      781 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
      841 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttccct
//
