LOCUS       sequence-60386-e         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: BIRC1A
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             15..37
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             90..110
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             491..508
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(561..579)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      575..622
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(746..765)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cctctagaaa taattttgtt taactttaag aaggagatat acatatgcac catcatcatc
       61 atcattcttc tggtgtagat ctgggtaccg agaacctgta cttccaatcc atgagggtga
      121 agaatctgaa gagcaggctg agaggaggta aaatgaggta ccaagaagag gaggctagac
      181 ttgcatcctt caggaactgg ccattttatg tccaagggat atccccttgt gtgctctcag
      241 aggctggctt tgtctttaca ggtaaacagg acacggtaca gtgtttttcc tgtggtggat
      301 gtttaggaaa ttgggaagaa ggagatgatc cttggaagga acatgccaaa tggttcccca
      361 aatgtgaatt tcttcggagt aagaaatcct cagaggaaat tacccagtat attcaaagct
      421 acaagtgaca gtaaaggtgg atacggatcc gaattcgagc tccgtcgaca agcttgcggc
      481 cgcactcgag caccaccacc accaccactg agatccggct gctaacaaag cccgaaagga
      541 agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa
      601 acgggtcttg aggggttttt tgctgaaagg aggaactata tccggattgg cgaatgggac
      661 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
      721 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
      781 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
      841 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
      901 tcgc
//
