LOCUS       sequence-60387-e         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PTK2BA
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             15..37
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             90..110
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 cctctagaaa taattttgtt taactttaag aaggagatat acatatgcac catcatcatc
       61 atcattcttc tggtgtagat ctgggtaccg agaacctgta cttccaatcc atgggaggtc
      121 cacagtatgg cattgcccgt gaagatgtgg tcctgaatcg tattcttggg gaaggctttt
      181 ttggggaggt ctatgaaggt gtctacacaa atcataaagg ggagaaaatc aatgtagctg
      241 tcaagacctg caagaaagac tgcactctgg acaacaagga gaagttcatg agcgaggcag
      301 tgatcatgaa gaacctcgac cacccgcaca tcgtgaagct gatcggcatc attgaagagg
      361 agcccacctg gatcatcatg gaattgtatc cctatgggga gctgggccac tacctggagc
      421 ggaacaagaa ctccctgaag gtgctcaccc tcgtgctgta ctcactgcag atatgcaaag
      481 ccatggccta cctggagagc atcaactgcg tgcacaggga cattgctgtc cggaacatcc
      541 tggtggcctc ccctgagtgt gtgaagctgg gggactttgg tctttcccgg tacattgagg
      601 acgaggacta ttacaaagcc tctgtgactc gtctccccat caaatggatg tccccagagt
      661 ccattaactt ccgacgcttc acgacagcca gtgacgtctg gatgttcgcc gtgtgcatgt
      721 gggagatcct gagctttggg aagcagccct tcttctggct ggagaacaag gatgtcatcg
      781 gggtgctgga gaaaggagac cggctgccca agcctgatct ctgtccaccg gtcctttata
      841 ccctcatgac ccgctgctgg gactacgacc ccagtgaccg gccccgcttc accgagctgg
      901 tgtgcagcct ca
//
