LOCUS       sequence-60388-e         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: GMPSA
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             15..37
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             90..110
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             764..781
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(834..852)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      848..895
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 cctctagaaa taattttgtt taactttaag aaggagatat acatatgcac catcatcatc
       61 atcattcttc tggtgtagat ctgggtaccg agaacctgta cttccaatcc atggaaggag
      121 ctgttgtcat tctggatgct ggtgctcagt acgggaaagt catagaccga agagtgaggg
      181 aactgttcgt gcagtctgaa attttcccct tggaaacacc agcatttgct ataaaggaac
      241 aaggattccg tgctattatc atctctggag gacctaattc tgtgtatgct gaagatgctc
      301 cctggtttga tccagcaata ttcactattg gcaagcctgt tcttggaatt tgctatggta
      361 tgcagatgat gaataaggta tttggaggta ctgtgcacaa aaaaagtgtc agagaagatg
      421 gagttttcaa cattagtgtg gataatacat gttcattatt caggggcctt cagaaggaag
      481 aagttgtttt gcttacacat ggagatagtg tagacaaagt agctgatgga ttcaaggttg
      541 tggcacgttc tggaaacata gtagcaggca tagcaaatga atctaaaaag ttatatggag
      601 cacagttcca ccctgaagtt ggccttacag aaaatggaaa agtaatactg aagaatttcc
      661 tttatgatat agctggatgc agtggaacct tcaccgtgtg acagtaaagg tggatacgga
      721 tccgaattcg agctccgtcg acaagcttgc ggccgcactc gagcaccacc accaccacca
      781 ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
      841 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa
      901 aggaggaact atatccgatg c
//
