LOCUS       sequence-60389-e       12818 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12818)
  AUTHORS   Semple-Rowland SL, Coggin WE, Geesey M, Eccles KS, Abraham L, 
            Pachigar K, Ludlow R, Khani SC, Smith WC
  TITLE     Expression characteristics of dual-promoter lentiviral vectors 
            targeting retinal photoreceptors and Muller cells.
  JOURNAL   Mol Vis. 2010 May 27;16:916-34.
  PUBMED    20517486
REFERENCE   2  (bases 1 to 12818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Vis."; 
            date: "2010-05-27"; volume: "16"; pages: "916-34"
COMMENT     Sequence Label: pFIN-NRLL-tdTOM
FEATURES             Location/Qualifiers
     source          1..12818
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     232..252
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    2474..2707
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2969..3086
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             5761..5775
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     regulatory      5782..5791
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5788..7218
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     polyA_signal    8171..8395
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8528..8547)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(9568..9585)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9739..10327)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9819..9838)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10498..11289,11290..11358))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11121..11140
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11359..11463)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11531..11549
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11600..11622)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11708..11846
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11761..11780
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gtggcccgag agctgcatcc ggagtatcta gatggagttc cgcgttacat aacttacggt
       61 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgactga
      121 tgttcccata gtaacgccaa tagggacttt ccattgacgc taatgggagt ttgttttggc
      181 accaaaatca acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg
      241 gcggtaggcg tgtactctag aaggtctata taagcagagc tcgtttagtg aaccgtttat
      301 actacttatc tggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
      361 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgctcaaag tagtgtgtgc
      421 ccgtctgttg tgtgactctg ccgcacccgt gtgcttttct ctctttttct ctgacactga
      481 ttttggtgca aaagctgttg ttcgtgtgag cccacagaaa attgtaagtg aagtggatta
      541 ggagcttaca ccgtcacgga gcaaattctg ccctcctaat cttctgctgg gctgtaatct
      601 gcaagacttt cccattttac ctcctttcta ttttaaacct gaatgcttaa tgctcctggt
      661 gggggtccat cctgctcctc attggctgag ggaggatttg ctggcactta aatggcaccc
      721 tctgctccgt gcctctgtgc tttttggttg ttgtgctttt acatttgccc ggagagagag
      781 aggaacctgg gtccatcacc accactgctg cactgggacc agcacgaggc actttaaagg
      841 tgagaaggag aacttcaaag ccccaaagct ttagaactca atcatcaacc tcagaactct
      901 tctatcaacc tcagaactca ccatccgacc ccaggactca cccaccgacc tcaaaactca
      961 tccattgacc tcagaactca tccaccgacc tcagaactca tccaccgacc tccagttttg
     1021 gctgcagagt gactggagcg cgtaactaga gatccctcag acccttttag tcagtgtgga
     1081 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagtaaagc cagaggagat
     1141 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
     1201 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
     1261 cgtcggtatt aagcggggga gaattagata aatgggaaaa aattcggtta aggccagggg
     1321 gaaagaaaca atataaacta aaacatatag tatgggcaag cagggagcta gaacgattcg
     1381 cagttaatcc tggcctttta gagacatcag aaggctgtag acaaatactg ggacagctac
     1441 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca atagcagtcc
     1501 tctattgtgt gcatcaaagg atagatgtaa aagacaccaa ggaagcctta gataagatag
     1561 aggaagagca aaacaaaagt aagaaaaagg cacagcaagc agcagctgac acaggaaaca
     1621 acagccaggt cagccaaaat taccctatag tgcagaacct ccaggggcaa atggtacatc
     1681 aggccatatc acctagaact ttaaatgcat gggtaaaagt agtagaagag aaggctttca
     1741 gcccagaagt aatacccatg ttttcagcat tatcagaagg agccacccca caagatttaa
     1801 ataccatgct aaacacagtg gggggacatc aagcagccat gcaaatgtta aaagagacca
     1861 tcaatgagga agctgcagaa tgggatagat tgcatccagt gcatgcaggg cctattgcac
     1921 caggccagat gagagaacca aggggaagtg acatagcagg aactactagc aaattaagag
     1981 aacaatttgg aaataataaa acaataatct ttaagcaatc ctcaggaggg gacccagaaa
     2041 ttgtaacgca cagttttaat tgtggagggg aatttttcta ctgtaattca acacaactgt
     2101 ttaatagtac ttggtttaat agtacttgga gtactgaagg gtcaaataac actgaaggaa
     2161 gtgacacaat cacactccca tgcagaataa aacaatttat aaacatgtgg caggaagtag
     2221 gaaaagcaat gtatgcccct cccatcagtg gacaaattag atgttcatca aatattactg
     2281 ggctgctatt aacaagagat ggtggtaata acaacaatgg gtccgagatc ttcagacctg
     2341 gaggaggcga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     2401 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     2461 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     2521 gcgcacggtc aatgacgctg acggtacagg ccagacaatt attgtctgat atagtgcagc
     2581 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     2641 ggggcatcaa acagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     2701 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     2761 atgctagttg gagtaataaa tctctggaac agatttggaa taacatgacc tggatggagt
     2821 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     2881 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggct tgcggccgga
     2941 attcacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca
     3001 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
     3061 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt
     3121 gggcggccgc gcgctaccgg actcagatct cgagctcaag cttcgaattc gatttggttc
     3181 ctggccttac attttccgct tcttttaaga aaataagcaa caggtcttgg tgaagagtaa
     3241 catttccatt cgaatagtga ctctatttca taaaccaaac gaaacgaaac gaaacgaaac
     3301 gaaacgaaac aaaacaaaac aaaacaaaac accaagagac ctgggcatgg gacacttgtg
     3361 atatctgacc taagccttct aatgtatgta gaggaagctt ggattccaaa tgtcctccaa
     3421 agctgggcat ggtggtgcgt gcctttacgc cctgtactag gaaggcacaa gcaggtagat
     3481 ctatgggttc aaggtcaatc tggtctacaa agagagttct aggacggcta gggctacaca
     3541 gagaaaccct gttttgaaaa ccaaaccaaa ccaaaccaat gtcctccaaa ggcatttgag
     3601 ttcaaaactt gtcattattg gaaggtggtg aaattttcaa gaggtggggc cctgtgggaa
     3661 gtcttctggt ctttgggttt tccctgaagg aagcaaaggc ctaacccctc cctggtcatc
     3721 ggttggtacc cttgagggaa gcaggagtcc tatccacttc ccttcccttt tctttttttt
     3781 ttttgtccca gtgataaggt gagtagcttt cagttgtgtc ctcgccataa cacttgactc
     3841 actacaggca atacatgatg ggaccaactg ctcactggct aggctctcca aggcagcaaa
     3901 ccaaagcaaa gcgctttccg attaagtcaa ttacttcagg tatgtattgg agcaattatg
     3961 ttagtgttag tcggtttttg ttattgtgac aaagtgcccg agaaagatca aaggagggga
     4021 gccttctttg ggctaatggc ttcagtttca gctgatggtt gtgggtgtcc tattgctttg
     4081 gtccaaaatc taacatcacg atggaaaatg tatagtgaag aagtaaagag agaaaagaga
     4141 gaggaagagg gggaagtgga ggtgggggag aaggagggat ggagggacac agacaaaatg
     4201 caccaatgaa ctaaggagaa gatacaccat tcaaacgcat gttttttcta gttaggccaa
     4261 ttcatactga cagccaattc agtatggatt cttcagctgg ttagttatgg ataagtttaa
     4321 cctctaataa tatctcagtg aaaactagct tttaaaatat acctatcgtc acggaaccat
     4381 agaaaactaa tgtagggtga cacacacaca cacacacaca cacaaacaca cactttgaga
     4441 ctgggttgaa ggtagccatc tgtggaccag tgggtctcaa acattagtgt gtttcagagc
     4501 tgttggaagg gtttattaaa cagcagctgt ctataaccta cttactgagt ttctggttct
     4561 gtgagtttga ggtaaagcaa aagaatttgt ttcttcaagt tcccaagggg tgatttctta
     4621 agactgtgga agccattgtt ccaacaggca gggaataccc tttaacttct ggagtttctt
     4681 ttatcacagc acctatttaa acagcttcag gcaggtttaa acatctcctt ggctaagtcc
     4741 tactatccct gcttcggccc ctactagggg tgacagctct aagaggcgtt aggcgattag
     4801 gctgaaaatg taggtcacac cccagccgtc tgggaatgag cgagagagga gaagagggac
     4861 tgagacaccg cacggggaaa aggtgagagg aagctgaggg ctcatgaggg cattcacaga
     4921 agtgtggtgg tgattggatt tcagcttctt ttagtctcca gatgaaagac cagagttggg
     4981 atctccgctt tttctatctc ttctctccag ctgggctgct tgtcttcggg atgggctatt
     5041 aagagcgagt ttcttcatgg aagctcatgg cttatctttc taatacagtg actctgtgag
     5101 gaataagacg agtcagggtg aaggtagggc aggggctgaa atgtgaggat gcttgaaaag
     5161 aagcagcagc cccaacaagc tggctgagga tgggctggga acgcgtatat gtctccttga
     5221 cacggagtct taaaagaggt tttgggtagt atgtctggac tcttaccact ctaagctttg
     5281 agtgattggg ctgagaattg tgtttccatg gaggggtggt gtttggggtc aacatggctc
     5341 tatattggaa accaagctca caacataagc aaggtctgat ataggaagct tctctgggga
     5401 agatgccgtt tgttttttta atttttggag aactgatagc acctgtcttt cactggcttc
     5461 tgagtccctg ccccaggcca atgagatatg caaataagcc cttcatctta ttggatgagg
     5521 ctcgaggacc acagatgatc tcagggaacc agtcctttaa gagtgtcctc tgggctctgt
     5581 gcccacagcc cccttgttct gaatacaggg acgacaccag cccctgctct atggagtatt
     5641 tagtctccag ggaagctgtg cctttctggt tctgacagtg actacgtcat ctctgccatt
     5701 acatcggatc caccggtcgc cgctagcgtt cgacccggga tcgatagttt aaacctgtac
     5761 gacgatgacg ataaggatcc cgccaccatg gtgagcaagg gcgaggaggt catcaaagag
     5821 ttcatgcgct tcaaggtgcg catggagggc tccatgaacg gccacgagtt cgagatcgag
     5881 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
     5941 ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc agttcatgta cggctccaag
     6001 gcgtacgtga agcaccccgc cgacatcccc gattacaaga agctgtcctt ccccgagggc
     6061 ttcaagtggg agcgcgtgat gaacttcgag gacggcggtc tggtgaccgt gacccaggac
     6121 tcctccctgc aggacggcac gctgatctac aaggtgaaga tgcgcggcac caacttcccc
     6181 cccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctccac cgagcgcctg
     6241 tacccccgcg acggcgtgct gaagggcgag atccaccagg ccctgaagct gaaggacggc
     6301 ggccactacc tggtggagtt caagaccatc tacatggcca agaagcccgt gcaactgccc
     6361 ggctactact acgtggacac caagctggac atcacctccc acaacgagga ctacaccatc
     6421 gtggaacagt acgagcgctc cgagggccgc caccacctgt tcctggggca tggcaccggc
     6481 agcaccggca gcggcagctc cggcaccgcc tcctccgagg acaacaacat ggccgtcatc
     6541 aaagagttca tgcgcttcaa ggtgcgcatg gagggctcca tgaacggcca cgagttcgag
     6601 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
     6661 accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt catgtacggc
     6721 tccaaggcgt acgtgaagca ccccgccgac atccccgatt acaagaagct gtccttcccc
     6781 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggtctggt gaccgtgacc
     6841 caggactcct ccctgcagga cggcacgctg atctacaagg tgaagatgcg cggcaccaac
     6901 ttcccccccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctccaccgag
     6961 cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc accaggccct gaagctgaag
     7021 gacggcggcc actacctggt ggagttcaag accatctaca tggccaagaa gcccgtgcaa
     7081 ctgcccggct actactacgt ggacaccaag ctggacatca cctcccacaa cgaggactac
     7141 accatcgtgg aacagtacga gcgctccgag ggccgccacc acctgttcct gtacggcatg
     7201 gacgagctgt acaagtaaga attcgaagct tgatccggct gctaacaaag cccgaaagga
     7261 gtcgacccgg gatccgcgga ctagtgaatt ccagctgagc gccggtcgct accattacca
     7321 gttggtctgg tgtcaaaaat aataataacc gggcgagggg gatctcgact ctagaagctt
     7381 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     7441 aaagggggga ctggaagggc taattcactc ccagtttcga gttggcgcgc ctgggagctc
     7501 acggggacag ccccccccaa agcccccagg gatgtaatta cgtccctccc ccgctagggg
     7561 gcagcagcga gccgcccggg gctccgctcc ggtccggcgc tccccccgca tccccgagcc
     7621 ggcagcgtgc ggggacagcc cgggcacggg gaaggtggca cgggatcgct ttcctctgaa
     7681 cgcttctcgc tgctctttga gcctgcagac acctggggga tacggggaaa aagctttagg
     7741 ctgaaagaga gatttagaat gacaggcgcg cctgggagct cacggggaca gcccccccca
     7801 aagcccccag ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg
     7861 ggctccgctc cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc
     7921 ccgggcacgg ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg
     7981 agcctgcaga cacctggggg atacggggaa aaagctttag gctgaaagag agatttagaa
     8041 tgacaggcgc gccaactcga attcattgca gactttcact gaaacgggtc tctctggtta
     8101 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     8161 taaagctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     8221 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     8281 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     8341 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     8401 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     8461 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     8521 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctgtgcggga
     8581 gaacggagtt ctattatgac tcaaatcagt ctccccaagc attcggggat cagagttttt
     8641 aaggataact tagtgtgtag ggggccagtg agttggagat gaaagcgtag ggagtcgaag
     8701 gtgtcctttt gcgccgagtc agttcctggg tgggggccac aagatcggat gagccagttt
     8761 atcaatccgg gggtgccagc tgatccatgg agtgcagggt ctgcaaaata tctcaagcac
     8821 tgattgatct taggttttac aatagtgatg ttaccccagg aacaatttgg ggaaggtcag
     8881 aatcttgtag cctgtagctg catgactcct aaaccataat ttcttttttg tttttttttt
     8941 tttatttttg agacagggtc tcactctgtc acctaggctg gagtgcagtg gtgcaatcac
     9001 agctcactgc agcctcaacg tcgtaagctc aagcgatcct cccacctcag cctgcctggt
     9061 agctgagact acaagcgacg ccccagttaa tttttgtatt tttggtagag gcagcgtttt
     9121 gccgtgtggc cctggctggt ctcgaactcc tgggctcaag tgatccagcc tcagcctccc
     9181 aaagtgctgg gacaaccggg gccagtcact gcacctggcc ctaaaccata atttctaatc
     9241 ttttggctaa tttgttagtc ctacaaaggc agtctagtcc ccaggcaaaa agggggtttg
     9301 tttcgggaaa gggctgttac tgtctttgtt tcaaactata aactaagttc ctcctaaact
     9361 tagttcggcc tacacccagg aatgaacaag gagagcttgg aggttagaag cacgatggaa
     9421 ttggttaggt cagatctctt tcactgtctg agttataatt ttgcaatggt ggttcaaaga
     9481 ctgcccgctt ctgacaccag tcgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     9541 gtttgcgtat tggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     9601 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     9661 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     9721 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     9781 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     9841 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     9901 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc
     9961 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
    10021 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
    10081 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
    10141 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
    10201 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
    10261 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
    10321 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
    10381 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
    10441 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
    10501 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
    10561 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    10621 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
    10681 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    10741 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    10801 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    10861 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    10921 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    10981 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    11041 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    11101 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    11161 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    11221 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    11281 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    11341 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    11401 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    11461 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
    11521 aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaactgc ctcgcgcgtt
    11581 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
    11641 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
    11701 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtactgg cttaactatg
    11761 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
    11821 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg
    11881 aagggcgatc ggtgcgggcc tcttcgctat tacgccagcg cggggaggca gagattgcag
    11941 taagctgaga tcgcagcact gcactccagc ctgggcgaca gagtaagact ctgtctcaaa
    12001 aataaaataa ataaatcaat cagatattcc aatcttttcc tttatttatt tatttatttt
    12061 ctattttgga aacacagtcc ttccttattc cagaattaca catatattct atttttcttt
    12121 atatgctcca gtttttttta gaccttcacc tgaaatgtgt gtatacaaaa tctaggccag
    12181 tccagcagag cctaaaggta aaaaataaaa taataaaaaa taaataaaat ctagctcact
    12241 ccttcacatc aaaatggaga tacagctgtt agcattaaat accaaataac ccatcttgtc
    12301 ctcaataatt ttaagcgcct ctctccacca catctaactc ctgtcaaagg catgtgcccc
    12361 ttccgggcgc tctgctgtgc tgccaaccaa ctggcatgtg gactctgcag ggtccctaac
    12421 tgccaagccc cacagtgtgc cctgaggctg ccccttcctt ctagcggctg cccccactcg
    12481 gctttgcttt ccctagtttc agttacttgc gttcagccaa ggtctgaaac taggtgcgca
    12541 cagagcggta agactgcgag agaaagagac cagctttaca gggggtttat cacagtgcac
    12601 cctgacagtc gtcagcctca cagggggttt atcacattgc accctgacag tcgtcagcct
    12661 cacagggggt ttatcacagt gcacccttac aatcattcca tttgattcac aattttttta
    12721 gtctctactg tgcctaactt gtaagttaaa tttgatcaga ggtgtgttcc cagaggggaa
    12781 aacagtatat acagggttca gtactatcgc atttcagg
//
