LOCUS       sequence-60390-e         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: NUDT16A
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             15..37
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             90..110
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             761..778
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(831..849)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      845..892
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 cctctagaaa taattttgtt taactttaag aaggagatat acatatgcac catcatcatc
       61 atcattcttc tggtgtagat ctgggtaccg agaacctgta cttccaatcc atggccggag
      121 cccgcaggct ggagctaggc gaggccctgg cgctggggtc gggctggcgt catgtgtgcc
      181 acgctctcct ctacgcgccg gaccctggga tgctcttcgg ccgcatcccg ctgcgctacg
      241 ccatactgat gcagatgcgc ttcgatggac gcctgggctt ccccggcgga ttcgtggaca
      301 cgcaggacag aagcctagag gacgggctga accgcgagct gcgcgaggag ctgggcgaag
      361 cggctgccgc tttccgcgtg gagcgcactg actaccgcag ctcccacgtc gggtcagggc
      421 cacgcgttgt ggcccacttc tatgccaagc gtctgacgct cgaggagctg ttggctgtgg
      481 aggccggcgc aacacgcgcc aaggaccacg ggctggaggt gctgggcctg gtgcgagtgc
      541 ccctgtatac cctgcgggat ggtgtaggag gcctgcctac cttcctggag aattccttta
      601 ttggctctgc gcgggagcag ttacttgaag ctctccagga cttgggactg ctgcagtctg
      661 gctctatttc aggccttaag attccagctc atcactgaca gtaaaggtgg atacggatcc
      721 gaattcgagc tccgtcgaca agcttgcggc cgcactcgag caccaccacc accaccactg
      781 agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
      841 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg
      901 aggaa
//
