LOCUS       sequence-60392-e         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: GLSA
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             15..37
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             90..110
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 cctctagaaa taattttgtt taactttaag aaggagatat acatatgcac catcatcatc
       61 atcattcttc tggtgtagat ctgggtaccg agaacctgta cttccaatcc atgattcctg
      121 actttatgtc ttttacatcg cacatcgacg agctgtacga gagtgctaaa aaacaatctg
      181 gcgggaaagt tgcggattat attccgcagc tggccaaatt tagtccggac ctgtggggag
      241 tctcagtgtg tacggtagat gggcagcgcc atagtaccgg tgatactaaa gttccgttct
      301 gcctgcaaag ctgtgtgaaa ccgctgaaat acgcgatcgc cgtcaacgac ctggggacag
      361 agtatgttca ccgctacgta ggcaaagaac cgtcggggct gcgctttaat aaactgtttc
      421 tgaacgaaga tgacaaaccg cataatccga tggtgaacgc gggcgccatt gtcgttacct
      481 ctctgatcaa acagggggtg aataacgcgg agaaattcga ttatgtaatg caatttctga
      541 ataaaatggc tggaaacgaa tacgtcggct tcagtaatgc cacgtttcag tcagagcgcg
      601 aaagcggtga tcgcaacttc gcaattgggt attatctgaa agaaaaaaaa tgctttcctg
      661 agggcaccga catggttggt atcctggatt tctactttca gctgtgttcc attgaagtga
      721 cttgcgaatc agcgtctgta atggccgcaa cactggcgaa tggaggcttc tgtccgatca
      781 cgggtgagcg tgttctgagt ccagaagctg tgcgcaacac cctgagcctg atgcactcat
      841 gcgggatgta tgacttttcc ggccaatttg ccttccatgt cggtctgccg gcgaaatcgg
      901 gagttgctgg cgg
//
