LOCUS       sequence-60395-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Ouyang X, Winbow VM, Patel LS, Burr GS, Mitchell CK, O'Brien J
  TITLE     Protein kinase A mediates regulation of gap junctions containing 
            connexin35 through a complex pathway.
  JOURNAL   Brain Res Mol Brain Res. 2005 Apr 27;135(1-2):1-11.
  PUBMED    15857663
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Brain Res 
            Mol Brain Res."; date: "2005-04-27"; volume: "135(1-2)"; pages: 
            "1-11"
COMMENT     Sequence Label: PCx35CT-S276A
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(26..49)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(278..289)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(320..364)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(380..397)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(407..424)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(472..494)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 tagcagccta ggtattaatc aattagtggt ggtggtggtg gtggtggtgc tcgagtgcgg
       61 ccgcaagctt gtatgtgtgt gtttgttaca cataagcaga gtcactggac tgagtacggc
      121 cgaaattagg cacactcatc ctcggcaagt ccttatttct gatctcataa atggccttcc
      181 gccgagcctg cactcctctc acggccgttt tgattttcct ccagcccagg tgattgagtt
      241 cttctagaat tcggatcccc gatatcgcca tggacccgcg tccctcaata ccggagccac
      301 caccggtacc cagatctggg ctgtccatgt gctggcgttc gaatttagca gcagcggttt
      361 ctttcatacc aattgcagta ctaccgcgtg gcaccagacc cgcggagtga tggtgatggt
      421 gatgaccaga accactagtt gaaccatccg attttggagg atggtcgcca ccaccaaacg
      481 tggcttgcca gccctgcaaa ggccatgcta tatacttgct ggatttcaag tacttatcaa
      541 tttgtgggat agcttcaata cgttttttaa aacaaactaa ttttgggaac gcatccaggc
      601 acattgggtc catgtataaa acaacatcaa gagcgtcata caacatgaag tcaggatggg
      661 ttacatgatc accatttaaa tatgttttat gacataaacg atcttcgaac attttcagca
      721 tttcaggtag cttgctaaga aaatcaactt tgagagtttc aaagtcttta ctatatgcaa
      781 ttctcgaaac accgtatcta atatccaaaa ccgctccttc aagcattgaa atctctgcac
      841 gctcttttgg
//
