LOCUS       sequence-60396-e         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FIGNL1A
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             15..37
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             90..110
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 cctctagaaa taattttgtt taactttaag aaggagatat acatatgcac catcatcatc
       61 atcattcttc tggtgtagat ctgggtaccg agaacctgta cttccaatcc atggttcctc
      121 ctatacccaa gcaagatggg ggagagcaga atggaggaat gcaatgtaag ccttatgggg
      181 caggacctac agaaccagca catccagttg atgagcgtct gaagaacttg gagccaaaga
      241 tgattgaact tattatgaat gagattatgg atcatggacc tccagtaaat tgggaagata
      301 ttgcaggagt agaatttgct aaagccacca taaaggaaat agttgtgtgg cccatgttga
      361 ggccagacat ctttactggt ttaaggggac cccctaaagg aattttgctc tttggtcctc
      421 ctgggactgg taaaactcta attggcaagt gcattgctag tcagtctggg gcaacattct
      481 ttagcatctc tgcttcatcc ttaacttcta aatgggtagg tgagggggag aaaatggtcc
      541 gtgcattgtt tgctgttgca aggtgtcagc aaccagctgt gatatttatt gacgaaattg
      601 attccttgtt atctcaacgg ggagatggtg agcatgaatc ttctagaagg ataaaaacag
      661 aatttttagt tcaattagat ggagcaacaa catcttctga agatcgtatc ctagtggtgg
      721 gagcaacaaa tcggccacaa gaaattgatg aggctgcccg gagaagattg gtgaaaaggc
      781 tttatattcc cctcccagaa gcttcagcca ggaaacagat agtaattaat ctaatgtcca
      841 aagagcagtg ttgcctcagt gaagaagaaa ttgaacagat tgtacagcag tctgatgcgt
      901 tttcaggagc agacatgaca cagctttgca gggaggc
//
