LOCUS       sequence-60400-e         462 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Non-integrating (episomal) expression of EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Okita K, Yamakawa T, Matsumura Y, Sato Y, Amano N, Watanabe A, 
            Goshima N, Yamanaka S
  TITLE     An Efficient Non-viral Method to Generate Integration-Free Human iPS
            Cells from Cord Blood and Peripheral Blood Cells.
  JOURNAL   Stem Cells. 2012 Nov 29. doi: 10.1002/stem.1293.
  PUBMED    23193063
REFERENCE   2  (bases 1 to 462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells.
            2012 Nov 29. doi: 10.1002/stem.1293."
COMMENT     Sequence Label: pCE-GFP
FEATURES             Location/Qualifiers
     source          1..462
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      15..24
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(52..71)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(133..155)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 tctgcccttg ctcaccatgg tggcgaattc tttgccaaaa tgatgagaca gcacaataac
       61 cagcacgttg cccaggagct gtaggaaaaa gaagaaggca tgaacatggt tagcagaggc
      121 tctagagccg ccggtcacac gccagaagcc gaaccccgcc ctgccccgtc ccccccgaag
      181 gcagccgtcc ccccgcggac agccccgagg ctggagaggg agaaggggac ggcggcgcgg
      241 cgacgcacga aggccctccc cgcccatttc cttcctgccg gcgccgcacc gcttcgcccc
      301 gcgcccgcta gagggggtgc ggcggcgcct cccagatttc ggctccgcac agatttggga
      361 caaaggaagt ccctgcgccc tctcgcacga ttaccataaa aggcaatggc tgcggctcgc
      421 cgcgcctcga cagccgccgg cgctccgggg gccgccgcgc cc
//
