LOCUS       sequence-60403-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TimeSTAMP method for tagging and visualization of newly synthesized 
            proteins. TimeSTAMPa cassette used for for low abundance proteins or
            proteins with slow ongoing synthesis rates.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Lin MZ, Tsien RY
  TITLE     TimeSTAMP tagging of newly synthesized proteins.
  JOURNAL   Curr Protoc Protein Sci. 2010 Feb;Chapter 26:Unit 26.5. doi: 
            10.1002/0471140864.ps2605s59.
  PUBMED    20155731
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Protoc
            Protein Sci."; date: "2010-02"; volume: "Chapter 26:Unit 26.5. doi";
            pages: " 10.1002/0471140864.ps2605s59"
COMMENT     Sequence Label: pCS6-mPSD95a-TSa-HA
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             91..114
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             811..837
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 caaatggggt cgacgctcta ccaggagttc gatgagatgg aagagtgctc tcagcactta
       61 cccggcgccg gcagtagtgg cgatatcatg gattacaagg atgacgacga taagggctct
      121 tccgggacag gctccggatc cggcactagt gcgcccatca cggcgtacgc ccagcagacg
      181 agaggcctcc tagggtgtat aatcaccagc ctgactggcc gggacaaaaa ccaagtggag
      241 ggtgaggtcc agatcgtgtc aactgctacc caaaccttcc tggcaacgtg catcaatggg
      301 gtatgctggg cagtctacca cggggccgga acgaggacca tcgcatcacc caagggtcct
      361 gtcatccaga tgtataccaa tgtggaccaa gaccttgtgg gctggcccgc tcctcaaggt
      421 tcccgctcat tgacaccctg tacctgcggc tcctcggacc tttacctggt cacgaggcac
      481 gccgatgtca ttcccgtgcg ccggcgaggt gatagcaggg gtagcctgct ttcgccccgg
      541 cccatttcct acttgaaagg ctcctcgggg ggtccgctgt tgtgccccgc gggacacgcc
      601 gtgggcctat tcagggccgc ggtgtgcacc cgtggagtgg ctaaagcggt ggactttatc
      661 cctgtggaga acctagagac aaccatgaga tccccggtgt tcacggacaa ctcctctcca
      721 ccagcagtca ccctgacgca cccaatcacc aaaatcgata gggaggttct ctaccaggag
      781 ttcgatgaga tggaagagtg ctctcagcac tatccctacg atgtgcccga ttacgctggc
      841 gcgcc
//
