LOCUS       sequence-60406-e         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: RRM2BA
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             15..37
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             90..110
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 cctctagaaa taattttgtt taactttaag aaggagatat acatatgcac catcatcatc
       61 atcattcttc tggtgtagat ctgggtaccg agaacctgta cttccaatcc atgtcagaca
      121 ccaacgaaag tgaaataaag tcaaatgaag agccactcct aagaaagagt tctcgccggt
      181 ttgtcatctt tccaatccag taccctgata tttggaaaat gtataaacag gcacaggctt
      241 ccttctggac agcagaagag gtcgacttat caaaggatct ccctcactgg aacaagctta
      301 aagcagatga gaagtacttc atctctcaca tcttagcctt ttttgcagcc agtgatggaa
      361 ttgtaaatga aaatttggtg gagcgcttta gtcaggaggt gcaggttcca gaggctcgct
      421 gtttctatgg ctttcaaatt ctcatcgaga atgttcactc agagatgtac agtttgctga
      481 tagacactta catcagagat cccaagaaaa gggaattttt atttaatgca attgaaacca
      541 tgccctatgt taagaaaaaa gcagattggg ccttgcgatg gatagcagat agaaaatcta
      601 cttttgggga aagagtggtg gcctttgctg ctgtagaagg agttttcttc tcaggatctt
      661 ttgctgctat attctggcta aagaagagag gtcttatgcc aggactcact ttttccaatg
      721 aactcatcag cagagatgaa ggacttcact gtgactttgc ttgcctgatg ttccaatact
      781 tagtaaataa gccttcagaa gaaagggtca gggagatcat tgttgatgct gtcaaaattg
      841 agcaggagtt tttaacagaa gccttgccag ttggcctcat tggaatgaat tgcattttga
      901 tgaaacagta cattgagttt g
//
