LOCUS       sequence-60408-e         970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 970)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DEADC1A
FEATURES             Location/Qualifiers
     source          1..970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             15..37
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             90..110
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             677..694
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(747..765)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      761..808
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(932..951)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cctctagaaa taattttgtt taactttaag aaggagatat acatatgcac catcatcatc
       61 atcattcttc tggtgtagat ctgggtaccg agaacctgta cttccaatcc atggaggaga
      121 ccgaaaagtg gatggaggag gcgatgcaca tggccaaaga agccctcgaa aatactgaag
      181 ttcctgttgg ctgtcttatg gtctacaaca atgaagttgt agggaagggg agaaatgaag
      241 ttaaccaaac caaaaatgct actcgacatg cagaaatggt ggccatcgat caggtcctcg
      301 attggtgtcg tcaaagtggc aagagtccct ctgaagtatt tgaacacact gtgttgtatg
      361 tcactgtgga gccgtgcatt atgtgtgcag ctgctctccg cctgatgaaa atcccgctgg
      421 ttgtatatgg ctgtcagaat gaacgatttg gtggttgtgg ctctgttcta aatattgcct
      481 ctgctgacct accaaacact gggagaccat ttcagtgtat ccctggatat cgggctgagg
      541 aagcagtgga aatgttaaag accttctaca aacaagaaaa tccaaatgca ccaaaatcga
      601 aagttcggaa atgacagtaa aggtggatac ggatccgaat tcgagctccg tcgacaagct
      661 tgcggccgca ctcgagcacc accaccacca ccactgagat ccggctgcta acaaagcccg
      721 aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc
      781 ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gattggcgaa
      841 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
      901 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
      961 gccacgttcg
//
