LOCUS       sequence-60412-e       10570 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10570)
  AUTHORS   Semple-Rowland SL, Coggin WE, Geesey M, Eccles KS, Abraham L, 
            Pachigar K, Ludlow R, Khani SC, Smith WC
  TITLE     Expression characteristics of dual-promoter lentiviral vectors 
            targeting retinal photoreceptors and Muller cells.
  JOURNAL   Mol Vis. 2010 May 27;16:916-34.
  PUBMED    20517486
REFERENCE   2  (bases 1 to 10570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Vis."; 
            date: "2010-05-27"; volume: "16"; pages: "916-34"
COMMENT     Sequence Label: pFIN-IRBP156-tdTOM
FEATURES             Location/Qualifiers
     source          1..10570
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     232..252
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    2474..2707
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2969..3086
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    3145..3201
                     /label=MCS
                     /note="multiple cloning site"
     CDS             3513..3527
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     regulatory      3534..3543
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3540..4970
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     polyA_signal    5923..6147
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6280..6299)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(7320..7337)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7491..8079)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7571..7590)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8250..9041,9042..9110))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8873..8892
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9111..9215)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9283..9301
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9352..9374)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9460..9598
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9513..9532
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gtggcccgag agctgcatcc ggagtatcta gatggagttc cgcgttacat aacttacggt
       61 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgactga
      121 tgttcccata gtaacgccaa tagggacttt ccattgacgc taatgggagt ttgttttggc
      181 accaaaatca acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg
      241 gcggtaggcg tgtactctag aaggtctata taagcagagc tcgtttagtg aaccgtttat
      301 actacttatc tggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
      361 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgctcaaag tagtgtgtgc
      421 ccgtctgttg tgtgactctg ccgcacccgt gtgcttttct ctctttttct ctgacactga
      481 ttttggtgca aaagctgttg ttcgtgtgag cccacagaaa attgtaagtg aagtggatta
      541 ggagcttaca ccgtcacgga gcaaattctg ccctcctaat cttctgctgg gctgtaatct
      601 gcaagacttt cccattttac ctcctttcta ttttaaacct gaatgcttaa tgctcctggt
      661 gggggtccat cctgctcctc attggctgag ggaggatttg ctggcactta aatggcaccc
      721 tctgctccgt gcctctgtgc tttttggttg ttgtgctttt acatttgccc ggagagagag
      781 aggaacctgg gtccatcacc accactgctg cactgggacc agcacgaggc actttaaagg
      841 tgagaaggag aacttcaaag ccccaaagct ttagaactca atcatcaacc tcagaactct
      901 tctatcaacc tcagaactca ccatccgacc ccaggactca cccaccgacc tcaaaactca
      961 tccattgacc tcagaactca tccaccgacc tcagaactca tccaccgacc tccagttttg
     1021 gctgcagagt gactggagcg cgtaactaga gatccctcag acccttttag tcagtgtgga
     1081 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagtaaagc cagaggagat
     1141 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
     1201 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
     1261 cgtcggtatt aagcggggga gaattagata aatgggaaaa aattcggtta aggccagggg
     1321 gaaagaaaca atataaacta aaacatatag tatgggcaag cagggagcta gaacgattcg
     1381 cagttaatcc tggcctttta gagacatcag aaggctgtag acaaatactg ggacagctac
     1441 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca atagcagtcc
     1501 tctattgtgt gcatcaaagg atagatgtaa aagacaccaa ggaagcctta gataagatag
     1561 aggaagagca aaacaaaagt aagaaaaagg cacagcaagc agcagctgac acaggaaaca
     1621 acagccaggt cagccaaaat taccctatag tgcagaacct ccaggggcaa atggtacatc
     1681 aggccatatc acctagaact ttaaatgcat gggtaaaagt agtagaagag aaggctttca
     1741 gcccagaagt aatacccatg ttttcagcat tatcagaagg agccacccca caagatttaa
     1801 ataccatgct aaacacagtg gggggacatc aagcagccat gcaaatgtta aaagagacca
     1861 tcaatgagga agctgcagaa tgggatagat tgcatccagt gcatgcaggg cctattgcac
     1921 caggccagat gagagaacca aggggaagtg acatagcagg aactactagc aaattaagag
     1981 aacaatttgg aaataataaa acaataatct ttaagcaatc ctcaggaggg gacccagaaa
     2041 ttgtaacgca cagttttaat tgtggagggg aatttttcta ctgtaattca acacaactgt
     2101 ttaatagtac ttggtttaat agtacttgga gtactgaagg gtcaaataac actgaaggaa
     2161 gtgacacaat cacactccca tgcagaataa aacaatttat aaacatgtgg caggaagtag
     2221 gaaaagcaat gtatgcccct cccatcagtg gacaaattag atgttcatca aatattactg
     2281 ggctgctatt aacaagagat ggtggtaata acaacaatgg gtccgagatc ttcagacctg
     2341 gaggaggcga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     2401 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     2461 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     2521 gcgcacggtc aatgacgctg acggtacagg ccagacaatt attgtctgat atagtgcagc
     2581 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     2641 ggggcatcaa acagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     2701 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     2761 atgctagttg gagtaataaa tctctggaac agatttggaa taacatgacc tggatggagt
     2821 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     2881 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggct tgcggccgga
     2941 attcacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca
     3001 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
     3061 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt
     3121 gggcggccgc gcgctaccgg actcagatct cgagctcaag cttcgaattc tgcagtcgac
     3181 ggtaccgcgg gcccgggatc cctacacaga catggctcag caagatgaga tgcaatgttc
     3241 cttccgggtc aaaccaccct ggcctcttct tcagagtcca gctcatgtgc ttgaattaga
     3301 caggattaaa ggcttagcag agctggaagc ctcacatcta actcccacat tgagccccag
     3361 accttctgtc tgcctgctaa gaagggcaag ggagagaggc agctgcagga ccacagcctt
     3421 gtacacacgg agctcttatc caggagccaa ggctctggga tccaccggtc gccgctagcg
     3481 ttcgacccgg gatcgatagt ttaaacctgt acgacgatga cgataaggat cccgccacca
     3541 tggtgagcaa gggcgaggag gtcatcaaag agttcatgcg cttcaaggtg cgcatggagg
     3601 gctccatgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     3661 gcacccagac cgccaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca
     3721 tcctgtcccc ccagttcatg tacggctcca aggcgtacgt gaagcacccc gccgacatcc
     3781 ccgattacaa gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
     3841 aggacggcgg tctggtgacc gtgacccagg actcctccct gcaggacggc acgctgatct
     3901 acaaggtgaa gatgcgcggc accaacttcc cccccgacgg ccccgtaatg cagaagaaga
     3961 ccatgggctg ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg
     4021 agatccacca ggccctgaag ctgaaggacg gcggccacta cctggtggag ttcaagacca
     4081 tctacatggc caagaagccc gtgcaactgc ccggctacta ctacgtggac accaagctgg
     4141 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgagcgc tccgagggcc
     4201 gccaccacct gttcctgggg catggcaccg gcagcaccgg cagcggcagc tccggcaccg
     4261 cctcctccga ggacaacaac atggccgtca tcaaagagtt catgcgcttc aaggtgcgca
     4321 tggagggctc catgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
     4381 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg cggccccctg cccttcgcct
     4441 gggacatcct gtccccccag ttcatgtacg gctccaaggc gtacgtgaag caccccgccg
     4501 acatccccga ttacaagaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
     4561 acttcgagga cggcggtctg gtgaccgtga cccaggactc ctccctgcag gacggcacgc
     4621 tgatctacaa ggtgaagatg cgcggcacca acttcccccc cgacggcccc gtaatgcaga
     4681 agaagaccat gggctgggag gcctccaccg agcgcctgta cccccgcgac ggcgtgctga
     4741 agggcgagat ccaccaggcc ctgaagctga aggacggcgg ccactacctg gtggagttca
     4801 agaccatcta catggccaag aagcccgtgc aactgcccgg ctactactac gtggacacca
     4861 agctggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gagcgctccg
     4921 agggccgcca ccacctgttc ctgtacggca tggacgagct gtacaagtaa gaattcgaag
     4981 cttgatccgg ctgctaacaa agcccgaaag gagtcgaccc gggatccgcg gactagtgaa
     5041 ttccagctga gcgccggtcg ctaccattac cagttggtct ggtgtcaaaa ataataataa
     5101 ccgggcgagg gggatctcga ctctagaagc ttggtacctt taagaccaat gacttacaag
     5161 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     5221 tcccagtttc gagttggcgc gcctgggagc tcacggggac agcccccccc aaagccccca
     5281 gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct
     5341 ccggtccggc gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg
     5401 gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag
     5461 acacctgggg gatacgggga aaaagcttta ggctgaaaga gagatttaga atgacaggcg
     5521 cgcctgggag ctcacgggga cagccccccc caaagccccc agggatgtaa ttacgtccct
     5581 cccccgctag ggggcagcag cgagccgccc ggggctccgc tccggtccgg cgctcccccc
     5641 gcatccccga gccggcagcg tgcggggaca gcccgggcac ggggaaggtg gcacgggatc
     5701 gctttcctct gaacgcttct cgctgctctt tgagcctgca gacacctggg ggatacgggg
     5761 aaaaagcttt aggctgaaag agagatttag aatgacaggc gcgccaactc gaattcattg
     5821 cagactttca ctgaaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     5881 gctaactagg gaacccactg cttaagcctc aataaagctc gactgtgcct tctagttgcc
     5941 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     6001 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     6061 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     6121 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     6181 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     6241 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     6301 cctttctcgc cacgttcgcc ggctgtgcgg gagaacggag ttctattatg actcaaatca
     6361 gtctccccaa gcattcgggg atcagagttt ttaaggataa cttagtgtgt agggggccag
     6421 tgagttggag atgaaagcgt agggagtcga aggtgtcctt ttgcgccgag tcagttcctg
     6481 ggtgggggcc acaagatcgg atgagccagt ttatcaatcc gggggtgcca gctgatccat
     6541 ggagtgcagg gtctgcaaaa tatctcaagc actgattgat cttaggtttt acaatagtga
     6601 tgttacccca ggaacaattt ggggaaggtc agaatcttgt agcctgtagc tgcatgactc
     6661 ctaaaccata atttcttttt tgtttttttt tttttatttt tgagacaggg tctcactctg
     6721 tcacctaggc tggagtgcag tggtgcaatc acagctcact gcagcctcaa cgtcgtaagc
     6781 tcaagcgatc ctcccacctc agcctgcctg gtagctgaga ctacaagcga cgccccagtt
     6841 aatttttgta tttttggtag aggcagcgtt ttgccgtgtg gccctggctg gtctcgaact
     6901 cctgggctca agtgatccag cctcagcctc ccaaagtgct gggacaaccg gggccagtca
     6961 ctgcacctgg ccctaaacca taatttctaa tcttttggct aatttgttag tcctacaaag
     7021 gcagtctagt ccccaggcaa aaagggggtt tgtttcggga aagggctgtt actgtctttg
     7081 tttcaaacta taaactaagt tcctcctaaa cttagttcgg cctacaccca ggaatgaaca
     7141 aggagagctt ggaggttaga agcacgatgg aattggttag gtcagatctc tttcactgtc
     7201 tgagttataa ttttgcaatg gtggttcaaa gactgcccgc ttctgacacc agtcgctgca
     7261 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attggcgctc ttccgcttcc
     7321 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     7381 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     7441 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     7501 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     7561 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     7621 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7681 tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7741 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7801 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     7861 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     7921 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     7981 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     8041 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     8101 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     8161 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     8221 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     8281 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     8341 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     8401 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     8461 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     8521 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     8581 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     8641 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     8701 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     8761 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     8821 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     8881 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     8941 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     9001 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     9061 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     9121 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     9181 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     9241 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     9301 ccctttcgtc ttcaagaact gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     9361 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     9421 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg
     9481 tagcgatagc ggagtgtact ggcttaacta tgcggcatca gagcagattg tactgagagt
     9541 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     9601 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     9661 attacgccag cgcggggagg cagagattgc agtaagctga gatcgcagca ctgcactcca
     9721 gcctgggcga cagagtaaga ctctgtctca aaaataaaat aaataaatca atcagatatt
     9781 ccaatctttt cctttattta tttatttatt ttctattttg gaaacacagt ccttccttat
     9841 tccagaatta cacatatatt ctatttttct ttatatgctc cagttttttt tagaccttca
     9901 cctgaaatgt gtgtatacaa aatctaggcc agtccagcag agcctaaagg taaaaaataa
     9961 aataataaaa aataaataaa atctagctca ctccttcaca tcaaaatgga gatacagctg
    10021 ttagcattaa ataccaaata acccatcttg tcctcaataa ttttaagcgc ctctctccac
    10081 cacatctaac tcctgtcaaa ggcatgtgcc ccttccgggc gctctgctgt gctgccaacc
    10141 aactggcatg tggactctgc agggtcccta actgccaagc cccacagtgt gccctgaggc
    10201 tgccccttcc ttctagcggc tgcccccact cggctttgct ttccctagtt tcagttactt
    10261 gcgttcagcc aaggtctgaa actaggtgcg cacagagcgg taagactgcg agagaaagag
    10321 accagcttta cagggggttt atcacagtgc accctgacag tcgtcagcct cacagggggt
    10381 ttatcacatt gcaccctgac agtcgtcagc ctcacagggg gtttatcaca gtgcaccctt
    10441 acaatcattc catttgattc acaatttttt tagtctctac tgtgcctaac ttgtaagtta
    10501 aatttgatca gaggtgtgtt cccagagggg aaaacagtat atacagggtt cagtactatc
    10561 gcatttcagg
//
