LOCUS       sequence-60414-e         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DDX52A
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             15..37
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             90..110
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             908..925
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cctctagaaa taattttgtt taactttaag aaggagatat acatatgcac catcatcatc
       61 atcattcttc tggtgtagat ctgggtaccg agaacctgta cttccaatcc atgaagataa
      121 acttcttgcg gaataaacac aaaattcacg tccaaggaac cgatcttcct gacccaattg
      181 ctacatttca gcaacttgac caggaatata aaatcaattc tcgactactt cagaacattc
      241 tagatgcagg tttccaaatg cctacgccaa tccaaatgca agccatccca gttatgctgc
      301 atggtcggga acttctggct tctgctccaa ctggatctgg aaaaacatta gcttttagca
      361 ttcctatttt aatgcagctg aaacaacccg caaataaagg cttcagagcc ctgattatat
      421 caccaacacg agaacttgcc agccagattc acagagagtt aataaaaatt tctgagggaa
      481 caggattcag gatacacatg atccacaaag cagcagtggc agccaagaaa tttggaccta
      541 aatcatctaa aaagtttgat attcttgtga ctactccaaa tcgactaatc tatttattaa
      601 agcaagatcc ccccggaatc gacctagcaa gtgttgagtg gcttgtagta gacgaatcag
      661 ataaactgtt tgaagatggc aaaactgggt tcagagacca gctggcttcc attttcctgg
      721 cctgcacatc ccacaaggtc cgaagagcta tgttcagtgc aacttttgca tatgatgttg
      781 aacagtggtg caaactcaac ctggacaatg tcatcagtgt gtccattgga gcaaggaatt
      841 cttgacagta aaggtggata cggatccgaa ttcgagctcc gtcgacaagc ttgcggccgc
      901 actcgagcac caccaccacc accactg
//
