LOCUS       sequence-60417-e         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CTPSA
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             49..66
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             91..111
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ccctctagaa ataattttgt ttaactttaa gaaggagata tacatatgca ccatcatcat
       61 catcattctt ctggtgtaga tctgggtacc gagaacctgt acttccaatc catgaagtac
      121 attctggtta ctggtggtgt tatatcagga attggaaaag gaatcattgc cagcagtgtg
      181 ggcacaatac tcaagtcatg tggtttacat gtaacttcaa tcaaaattga cccctacatt
      241 aacattgatg caggaacatt ctctccttat gagcatggtg aggtttttgt gctggatgat
      301 ggtggggaag tagaccttga cctgggtaac tatgagcggt tccttgacat ccgcctcacc
      361 aaggacaata atctgaccac tggaaagata taccagtatg tcattaacaa ggaacggaaa
      421 ggagattact tggggaaaac tgtccaagtt gtccctcata tcacagatgc aatccaggag
      481 tgggtgatga gacaggcgtt aatacctgta gatgaagatg gcctggaacc tcaagtgtgt
      541 gttattgagc ttggtggaac cgtgggggac atagaaagca tgccctttat tgaggccttc
      601 cgtcagttcc aattcaaggt caaaagagag aacttttgta acatccacgt cagtctagtt
      661 ccccagccaa gttcaacagg ggaacagaag actaaaccta cccagaatag tgttcgggaa
      721 cttagaggac ttgggctttc cccagatctg gttgtatgca ggtgctcaaa tccacttgac
      781 acatcagtga aggagaaaat atcaatgt
//
